Abiko T, Sekino H
Kidney Research Laboratory, Sendai, Japan.
Chem Pharm Bull (Tokyo). 1990 Feb;38(2):487-91. doi: 10.1248/cpb.38.487.
Human splenin (hSP) was synthesized by assembling eight peptide fragments followed by deprotection with 1M trifluoromethanesulfonic acid-thioanisole (molar ratios, 1:1) in trifluoroacetic acid in the presence of m-cresol and dimethylselenium. Finally, the deprotected peptide was incubated with dithiothreitol to reduce sulfoxide on the methionine side chain. Incubation of peripheral lymphocytes isolated from uremic patients with the synthetic hSP showed an enhancing effect on the reduced B-lymphocytes, but had no restoring effect on the impaired blastogenic response of T-lymphocytes.
人脾素（hSP）通过组装八个肽片段合成，随后在间甲酚和二甲基硒存在下，于三氟乙酸中用1M三氟甲磺酸 - 硫代苯甲醚（摩尔比1:1）进行脱保护。最后，将脱保护的肽与二硫苏糖醇一起孵育，以还原甲硫氨酸侧链上的亚砜。用合成的hSP孵育人尿毒症患者分离的外周淋巴细胞，结果显示对减少的B淋巴细胞有增强作用，但对受损的T淋巴细胞增殖反应无恢复作用。
