使用二维红外光谱对肽进行光学指纹识别：原理验证。

Optical fingerprinting of peptides using two-dimensional infrared spectroscopy: proof of principle.

作者信息

Fournier Frédéric, Gardner Elizabeth M, Guo Rui, Donaldson Paul M, Barter Laura M C, Palmer D Jason, Barnett Chris J, Willison Keith R, Gould Ian R, Klug David R

机构信息

Molecular Dynamics Research Group, Department of Chemistry, Imperial College London, London SW7 2AZ, UK.

出版信息

Anal Biochem. 2008 Mar 15;374(2):358-65. doi: 10.1016/j.ab.2007.11.009. Epub 2007 Nov 13.

DOI:10.1016/j.ab.2007.11.009

PMID:18062912

Abstract

We employ a particular form of two-dimensional infrared four-wave mixing (2DIR FWM) as a vibrational spectroscopic tool to quantify the amino acid content of a number of peptides. Vibrational features corresponding to ring modes of the aromatic groups of phenylalanine (Phe) and tyrosine (Tyr), as well as a methylene mode that is used as an internal reference, are identified. We show that the ratios of the integrated intensities, and the amplitudes, of the aromatic peaks of Phe and Tyr relative to the methylene integrated intensity, and amplitude, are proportional to the actual ratio of Phe and Tyr to CH(2) in the samples within a precision of +/-12.5%. This precision is shown to be sufficient to use this form of 2DIR spectroscopy as a possible proteins fingerprinting tool.

摘要

我们采用一种特殊形式的二维红外四波混频（2DIR FWM）作为振动光谱工具，来定量多种肽的氨基酸含量。识别出了与苯丙氨酸（Phe）和酪氨酸（Tyr）芳香基团的环模式相对应的振动特征，以及用作内部参考的亚甲基模式。我们表明，Phe和Tyr芳香峰的积分强度与振幅相对于亚甲基积分强度与振幅的比值，在±12.5%的精度范围内与样品中Phe和Tyr与CH₂的实际比值成正比。结果表明，这种精度足以将这种形式的2DIR光谱用作一种可能的蛋白质指纹识别工具。