利用光镊融合乳液微滴对单细胞进行空间选择性采样：一种新型单细胞蛋白质组学工具。
Spatially selective sampling of single cells using optically trapped fusogenic emulsion droplets: a new single-cell proteomic tool.
作者信息
Lanigan Peter M P, Chan Karen, Ninkovic Tanya, Templer Richard H, French P M W, de Mello A J, Willison K R, Parker P J, Neil M A A, Ces Oscar, Klug D R
机构信息
The Single Cell Proteomics Group, Chemical Biology Centre (CBC), Imperial College London, Exhibition Road, London SW7 2AZ, UK.
出版信息
J R Soc Interface. 2008 Oct 6;5 Suppl 2(Suppl 2):S161-8. doi: 10.1098/rsif.2008.0249.focus.
We present a platform for the spatially selective sampling of the plasma membrane of single cells. Optically trapped lipid-coated oil droplets (smart droplet microtools, SDMs), typically 0.5-5 microm in size, composed of a hexadecane hydrocarbon core and fusogenic lipid outer coating (mixture of 1,2-dioleoyl-phosphatidylethanolamine and 1,2-dioleoyl-sn-glycero-3-phosphatidylcholine) were brought into controlled contact with target colon cancer cells leading to the formation of connecting membrane tethers. Material transfer from the cell to the SDM across the membrane tether was monitored by tracking membrane-localized enhanced green fluorescent protein.
我们展示了一个用于单细胞质膜空间选择性采样的平台。光学捕获的脂质包被油滴（智能液滴微工具，SDMs），通常尺寸为0.5 - 5微米，由十六烷烃核心和促融合脂质外层（1,2 - 二油酰基磷脂酰乙醇胺和1,2 - 二油酰基 - sn - 甘油 - 3 - 磷脂酰胆碱的混合物）组成，使其与目标结肠癌细胞进行受控接触，导致形成连接膜系链。通过追踪膜定位的增强型绿色荧光蛋白来监测物质从细胞通过膜系链转移到SDM的过程。
Zotero 插件