Tsuzuki Joyce K, Svidzinski Terezinha I E, Shinobu Cristiane S, Silva Luiz F A, Rodrigues-Filho Edson, Cortez Diógenes A G, Ferreira Izabel C P
Departamento de Farmácia e Farmacologia, Universidade Estadual de Maringá, Maringá, PR, Brazil.
An Acad Bras Cienc. 2007 Dec;79(4):577-83. doi: 10.1590/s0001-37652007000400002.
Extracts from the dried pericarp of Sapindus saponaria L. (Sapindaceae) fruits were investigated for their antifungal activity against clinical isolates of yeasts Candida albicans and C. non-albicans from vaginal secretions of women with Vulvovaginal Candidiasis. Four clinical isolates of C. albicans, a single clinical isolated of each of the species C. parapsilosis, C. glabrata, C. tropicalis, and the strain of C. albicans ATCC 90028 were used. The hydroalcoholic extract was bioactivity-directed against a clinical isolate of C. parapsilosis, and showed strong activity. The n-BuOH extract and one fraction showed strong activity against all isolates tested. Further column-chromatography on silica gel separation of this fraction afforded two pure triterpene acetylated saponins: 3-O-(4-acetyl-beta-D-xylopyranosyl)-(1->3)-alpha-L-rhamnopyranosyl-(1->2)-alpha-L-arabinopyranosyl-hederagenin (1) and 3-O-(3,4-di-acetyl-beta-D-xylopyranosyl)-(1->3)-alpha-L-rhamnopyranosyl-(1->2)-alpha-L-arabynopyranosyl-hederagenin (2). The structures of the compounds were based on spectral data ((1)H and 13C NMR, HSQC, HMBC and MS), and on with literature. The saponins isolated showed strong activity against C. parapsilosis.
对无患子科植物无患子干燥果皮的提取物进行了研究，考察其对来自外阴阴道念珠菌病女性阴道分泌物中白色念珠菌和非白色念珠菌临床分离株的抗真菌活性。使用了4株白色念珠菌临床分离株，以及近平滑念珠菌、光滑念珠菌、热带念珠菌各1株临床分离株和白色念珠菌ATCC 90028菌株。水醇提取物针对近平滑念珠菌的1株临床分离株进行生物活性导向研究，显示出较强活性。正丁醇提取物及其一个馏分对所有测试分离株均显示出较强活性。对该馏分进一步进行硅胶柱色谱分离，得到两种纯的三萜乙酰化皂苷：3-O-(4-乙酰基-β-D-吡喃木糖基)-(1→3)-α-L-吡喃鼠李糖基-(1→2)-α-L-阿拉伯吡喃糖基常春藤皂苷元(1)和3-O-(3,4-二乙酰基-β-D-吡喃木糖基)-(1→3)-α-L-吡喃鼠李糖基-(1→2)-α-L-阿拉伯吡喃糖基常春藤皂苷元(2)。化合物的结构基于光谱数据(1H和13C NMR、HSQC、HMBC和MS)以及文献确定。分离得到的皂苷对近平滑念珠菌显示出较强活性。
