Obert Bohus, Middlefell-Williams Jill, Millam Steve
Scottish Crop Research Institute, Invergowrie, Dundee, DD2 5DA, Scotland, UK.
Biotechnol Lett. 2008 May;30(5):945-9. doi: 10.1007/s10529-007-9618-9. Epub 2007 Dec 8.
Bombardment of intact anthers of commercial barley (Hordeum vulgare) varieties resulted in 0.5-1.0% of transformed microspores of which 20-40% continued in androgenic development (0.2% of all bombarded microspores). Using a system based on bombardment of anthers is therefore likely to be more technically efficient than the use of a microspore isolation, transformation and regeneration system. Bombardment of anthers has a number of technical and scientific advantages over existing systems for gene transfer and can be considered as a alternative method to existing methods for genetic transformation in barley.
对商业大麦（大麦属）品种的完整花药进行轰击，产生了0.5 - 1.0%的转化小孢子，其中20 - 40%继续进行雄核发育（占所有轰击小孢子的0.2%）。因此，使用基于花药轰击的系统在技术上可能比使用小孢子分离、转化和再生系统更有效。与现有的基因转移系统相比，花药轰击具有许多技术和科学优势，可被视为大麦遗传转化现有方法的替代方法。
