Goodpaster Tracy, Legesse-Miller Aster, Hameed Meera R, Aisner Seena C, Randolph-Habecker Julie, Coller Hilary A
Department of Molecular Biology, Lewis Thomas Laboratory, Room 140, Princeton University, Princeton, NJ 08544, USA.
J Histochem Cytochem. 2008 Apr;56(4):347-58. doi: 10.1369/jhc.7A7287.2007. Epub 2007 Dec 10.
Fibroblasts are critical for tissue homeostasis, and their inappropriate proliferation and activation can result in common and debilitating conditions including fibrosis and cancer. We currently have a poor understanding of the mechanisms that control the growth and activation of fibroblasts in vivo, in part because of a lack of suitable fibroblast markers. We have taken advantage of an antibody previously shown to stain stromal cells in frozen tissues (TE-7) and identified conditions in which it can be used to stain fibroblasts and myofibroblasts in the paraffin-embedded tissue samples routinely collected for pathological analysis. We show that this antibody recognizes growing and quiescent fibroblasts and myofibroblasts by immunohistochemistry, immunofluorescence, and ELISA assays. We also present its staining patterns in normal tissue samples and in breast tumors.
成纤维细胞对组织稳态至关重要，其不适当的增殖和激活会导致包括纤维化和癌症在内的常见且使人衰弱的病症。目前，我们对体内控制成纤维细胞生长和激活的机制了解甚少，部分原因是缺乏合适的成纤维细胞标志物。我们利用了一种先前已证明可对冷冻组织中的基质细胞进行染色的抗体（TE-7），并确定了在常规收集用于病理分析的石蜡包埋组织样本中，利用该抗体对成纤维细胞和平滑肌肌动蛋白阳性成纤维细胞进行染色的条件。我们通过免疫组织化学、免疫荧光和酶联免疫吸附测定法表明，该抗体可识别增殖期和静止期的成纤维细胞和平滑肌肌动蛋白阳性成纤维细胞。我们还展示了其在正常组织样本和乳腺肿瘤中的染色模式。
