Woodtayakorn J, Punnarugsa V
Department of Microbiology, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand.
Asian Pac J Allergy Immunol. 1991 Dec;9(2):121-4.
133 nasopharyngeal aspirates (NPA) were simultaneously tested for the presence of respiratory syncytial virus (RSV) by conventional cell culture (CCC), shell vial centrifugation culture (SVC), immunofluorescence assay (IFA) and biotin-avidin enzyme linked immunosorbent assay (B-A ELISA). These yielded positive results in 32(24%), 45(33.8%), 36(27%) and 40(30%) of specimens, respectively. Specimens positive by IFA and B-A ELISA were all also positive by SVC. The sensitivity of CCC, IFA, and B-A ELISA comparing to SVC was 71%, 80%, and 88.9%, respectively. For rapid detection of RSV, we recommend the SVC method where a cell culture laboratory is available and the B-A ELISA method where a cell culture laboratory is not available.
采用常规细胞培养（CCC）、空斑小室离心培养（SVC）、免疫荧光测定法（IFA）和生物素-抗生物素蛋白酶联免疫吸附测定法（B-A ELISA），同时对133份鼻咽抽吸物（NPA）进行呼吸道合胞病毒（RSV）检测。这些方法分别在32份（24%）、45份（33.8%）、36份（27%）和40份（30%）标本中检测出阳性结果。免疫荧光测定法和生物素-抗生物素蛋白酶联免疫吸附测定法检测出的阳性标本，空斑小室离心培养法检测也均为阳性。与空斑小室离心培养法相比，常规细胞培养法、免疫荧光测定法和生物素-抗生物素蛋白酶联免疫吸附测定法的灵敏度分别为71%、80%和88.9%。对于呼吸道合胞病毒的快速检测，我们建议，在有细胞培养实验室的情况下采用空斑小室离心培养法，在没有细胞培养实验室的情况下采用生物素-抗生物素蛋白酶联免疫吸附测定法。
