[鼻咽分泌物中合胞体呼吸道病毒检测方法的评估]
[Evaluation of methods for the detection of syncytial respiratory virus in nasopharyngeal secretions].
作者信息
Buesa F J, García-Verdú R, Pastor M, Escribano A
机构信息
Departamento de Microbiología, Facultad de Medicina, Universidad de Valencia.
出版信息
Enferm Infecc Microbiol Clin. 1990 Feb;8(2):78-81.
The screening for respiratory syncytial virus (RSV) in nasopharyngeal secretions with enzyme immunoassay (ELISA) and indirect immunofluorescence (IIF) has been evaluated in infants and young children with acute respiratory infection. Both methods were compared with viral isolation in HEp-2 cells and the investigation of fluorescent foci in cell cultures inoculated by centrifugation. 226 samples were evaluated by IFF, 182 of which were also evaluated by ELISA while 158 were inoculated into cell cultures. 20.35% of samples were positive with IFF and 19.23% with ELISA. Isolation of RSV was obtained in 25 of the samples inoculated into HEp-2 cells (15.8%). The cytopathic effect took a mean of 5.4 days to develop. The investigation of fluorescent foci in centrifugated cultures allowed to detect 76% of positive samples 24 hours after centrifugation and 84% of positive samples 48 hours after it. Considering the viral isolation as the reference method, IIF and ELISA had a 88% and 76% sensitivity, respectively, with very similar specifities (90.2% and 91.7%).
采用酶免疫测定法（ELISA）和间接免疫荧光法（IIF）对患有急性呼吸道感染的婴幼儿鼻咽分泌物中的呼吸道合胞病毒（RSV）进行了筛查评估。将这两种方法与在HEp-2细胞中的病毒分离以及对通过离心接种的细胞培养物中的荧光灶进行的研究作了比较。通过IIF评估了226份样本，其中182份样本也通过ELISA进行了评估，158份样本接种到了细胞培养物中。IIF检测出20.35%的样本呈阳性，ELISA检测出19.23%的样本呈阳性。在接种到HEp-2细胞中的样本中有25份（15.8%）分离出了RSV。细胞病变效应平均需要5.4天出现。对离心培养物中的荧光灶进行的研究能够在离心后24小时检测出76%的阳性样本，在离心后48小时检测出84%的阳性样本。以病毒分离作为参考方法，IIF和ELISA的敏感性分别为88%和76%，特异性非常相似（分别为90.2%和91.7%）。
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