Yamakawa Makoto, Yanase Tohru, Kato Tomoko, Tsuda Tomoyuki
Kyushu Research Station, National Institute of Animal Health, 2702, Chuzan, Kagoshima 891-0105, Japan.
Vet Microbiol. 2008 May 25;129(1-2):40-7. doi: 10.1016/j.vetmic.2007.11.001. Epub 2007 Nov 9.
The sequences of a small RNA segment of Aino virus isolates were analyzed to define the molecular epidemiology and genetic relationships to other species in the genus Orthobunyavirus in the family Bunyaviridae. The nucleotide and amino acid sequences of the segment were highly conserved among strains isolated from 1964 to 2002 in Japan. These Japanese isolates were segregated into two distinct lineages, one containing the prototype strain JaNAr28 isolated in 1964 and the other containing strains isolated after 1986, by phylogenetic analysis based on the nucleocapsid gene sequences. Japanese strains isolated after 1986 were rather more closely related to Kaikalur virus isolated in India in 1971 than to strain JaNAr28. On the other hand, an Australian strain, B7974, was closely related to Peaton virus. The B7974 strain might have been generated by inter-serotype genetic reassortment between Aino and Peaton viruses in Australia during their evolution. However, recent Aino virus strains isolated in Japan appear to be genetically stable.
对艾诺病毒分离株的一个小RNA片段序列进行了分析，以确定其分子流行病学以及与布尼亚病毒科正布尼亚病毒属其他物种的遗传关系。在1964年至2002年期间从日本分离出的毒株中，该片段的核苷酸和氨基酸序列高度保守。通过基于核衣壳基因序列的系统发育分析，这些日本分离株被分为两个不同的谱系，一个包含1964年分离出的原型毒株JaNAr28，另一个包含1986年以后分离出的毒株。1986年以后在日本分离出的毒株与1971年在印度分离出的凯卡卢尔病毒的亲缘关系，比与毒株JaNAr28的亲缘关系更为密切。另一方面，一株澳大利亚毒株B7974与皮顿病毒密切相关。B7974毒株可能是在澳大利亚艾诺病毒和皮顿病毒进化过程中通过血清型间基因重配产生的。然而，最近在日本分离出的艾诺病毒毒株似乎在遗传上是稳定的。
