Ho Joanna W, Hershkovitz Oren, Peiris Malik, Zilka Alon, Bar-Ilan Ahuva, Nal Beatrice, Chu Kid, Kudelko Mateusz, Kam Yiu Wing, Achdout Hagit, Mandelboim Michal, Altmeyer Ralf, Mandelboim Ofer, Bruzzone Roberto, Porgador Angel
Shraga Segal Department of Microbiology and Immunology, Faculty of Health Sciences, Ben-Gurion University of the Negev, Beer-Sheva 84105, Israel.
J Virol. 2008 Feb;82(4):2028-32. doi: 10.1128/JVI.02065-07. Epub 2007 Dec 12.
Antiviral immune defenses involve natural killer (NK) cells. We previously showed that the NK-activating receptor NKp44 is involved in the functional recognition of H1-type influenza virus strains by NK cells. In the present study, we investigated the interaction of NKp44 and the hemagglutinin of a primary influenza virus H5N1 isolate. Here we show that recombinant NKp44 recognizes H5-expressing cells and specifically interacts with soluble H5 hemagglutinin. H5-pseudotyped lentiviral particles bind to NK cells expressing NKp44. Following interaction with target cells expressing H5, pseudotyped lentiviral particles, or membrane-associated H5, NK cells show NKp44-mediated induced activity. These findings indicate that NKp44-H5 interactions induce functional NK activation.
抗病毒免疫防御涉及自然杀伤（NK）细胞。我们之前表明，NK激活受体NKp44参与NK细胞对H1型流感病毒株的功能识别。在本研究中，我们调查了NKp44与一株原发性H5N1流感病毒血凝素之间的相互作用。在此我们表明，重组NKp44识别表达H5的细胞，并与可溶性H5血凝素特异性相互作用。H5假型慢病毒颗粒与表达NKp44的NK细胞结合。在与表达H5的靶细胞、假型慢病毒颗粒或膜相关H5相互作用后，NK细胞表现出NKp44介导的诱导活性。这些发现表明，NKp44-H5相互作用诱导功能性NK激活。
