Ibarra Ingrid, Erlich Yaniv, Muthuswamy Senthil K, Sachidanandam Ravi, Hannon Gregory J
Watson School of Biological Sciences, Howard Hughes Medical Institute, Cold Spring Harbor Laboratory, Cold Spring Harbor, New York 11724, USA.
Genes Dev. 2007 Dec 15;21(24):3238-43. doi: 10.1101/gad.1616307.
microRNA (miRNA) expression profiles are often characteristic of specific cell types. The mouse mammary epithelial cell line, Comma-Dbeta, contains a population of self-renewing progenitor cells that can reconstitute the mammary gland. We purified this population and determined its miRNA signature. Several microRNAs, including miR-205 and miR-22, are highly expressed in mammary progenitor cells, while others, including let-7 and miR-93, are depleted. Let-7 sensors can be used to prospectively enrich self-renewing populations, and enforced let-7 expression induces loss of self-renewing cells from mixed cultures.
微小RNA（miRNA）表达谱通常是特定细胞类型的特征。小鼠乳腺上皮细胞系Comma-Dbeta包含一群能够重建乳腺的自我更新祖细胞。我们纯化了这群细胞并确定了其miRNA特征。包括miR-205和miR-22在内的几种微小RNA在乳腺祖细胞中高度表达，而包括let-7和miR-93在内的其他一些微小RNA则减少。Let-7传感器可用于前瞻性富集自我更新群体，并且强制表达let-7会导致混合培养物中自我更新细胞的丢失。
