Mikkelsen Ulla Ramer, Helmark Ida Carøe, Kjaer Michael, Langberg Henning
Institute of Sports Medicine, Bispebjerg Hospital, DK-2400 Copenhagen NV, Copenhagen, Denmark.
J Appl Physiol (1985). 2008 Feb;104(2):534-7. doi: 10.1152/japplphysiol.01016.2007. Epub 2007 Dec 13.
Prostaglandins are known to be involved in the regulation of local blood flow within human skeletal muscles during exercise, and the concentration of prostaglandins increases locally and systemically in response to exercise. The systemic release of prostaglandins can be inhibited by oral intake of nonsteroidal anti-inflammatory drugs (NSAIDs). However, to study the local role of prostaglandins, the formation of prostaglandins within the tissue must be controlled. Microdialysis enables determination of local concentrations of water-soluble substances within the tissue. In the present study, the microdialysis method was used to infuse NSAIDs locally into human skeletal muscles producing a local block of prostaglandin formation. In addition, the graded blockade at various distances from the infusion site within the muscle during rest, exercise and recovery was determined. Microdialysis was performed in thigh muscles (vastus lateralis muscle) in six healthy men. One of the microdialysis catheters was used to block prostaglandin synthesis by infusion of the NSAID indomethacin. Additional catheters were placed 1 and 4 cm away from the infusion and in the contralateral leg (working control). Following 2 h of rest, the subjects performed 200 maximal eccentric contractions with each leg followed by 3 h of rest. The study revealed that infusion of NSAID reduced local prostaglandin E(2) concentration by approximately 30-50% (4 cm away from the infusion) and 85% (1 cm away from the infusion) compared with the contralateral (unblocked) thigh muscle. In conclusion, the present study shows that infusion of NSAIDs into human muscle via microdialysis catheters results in a graded blockade of prostaglandin synthesis.
已知前列腺素参与运动期间人体骨骼肌局部血流的调节，并且前列腺素的浓度会因运动而在局部和全身升高。口服非甾体抗炎药（NSAIDs）可抑制前列腺素的全身释放。然而，为了研究前列腺素的局部作用，必须控制组织内前列腺素的形成。微透析能够测定组织内水溶性物质的局部浓度。在本研究中，采用微透析方法将NSAIDs局部注入人体骨骼肌，以局部阻断前列腺素的形成。此外，还测定了在休息、运动和恢复期间，肌肉内距注入部位不同距离处的分级阻断情况。对6名健康男性的大腿肌肉（股外侧肌）进行了微透析。其中一根微透析导管用于通过注入非甾体抗炎药吲哚美辛来阻断前列腺素合成。在距注入部位1 cm和4 cm处以及对侧腿部（有效对照）放置额外的导管。休息2小时后，受试者每条腿进行200次最大离心收缩，随后休息3小时。研究表明，与对侧（未阻断）大腿肌肉相比，注入NSAIDs可使局部前列腺素E2浓度降低约30 - 50%（距注入部位4 cm处）和85%（距注入部位1 cm处）。总之，本研究表明通过微透析导管将NSAIDs注入人体肌肉会导致前列腺素合成的分级阻断。
