Institute of Sports Medicine, Department of Orthopedic Surgery M81, Bispebjerg Hospital and Center for Healthy Aging, Faculty of Health Sciences, University of Copenhagen, Bispebjerg Bakke 23,Copenhagen, Denmark.
J Appl Physiol (1985). 2012 Oct;113(7):1040-7. doi: 10.1152/japplphysiol.01463.2011. Epub 2012 Jul 5.
Menopause is associated with loss of collagen content in the skin and tendon as well as accumulation of noncontractile tissue in skeletal muscle. The relative role of hormones and physical activity on these changes is not known. Accordingly, in a randomized, controlled, crossover study we investigated effects of transdermal estrogen replacement therapy (ERT) on type I collagen synthesis in tendon and skeletal muscle in 11 postmenopausal women. Patches with estrogen (Evorel) were placed on the skin above the patellar tendons and compared with no patch (control period). On day 2 all subjects performed one-legged exercise, and thereafter the exercised leg (EX leg) was compared with the nonexercised leg (Rest leg). Microdialysis catheters were placed in front of the patellar tendons and in the vastus lateralis muscle of both legs at days 3 and 5. The collected dialysate was analyzed for procollagen type I NH(2)-terminal propeptide (PINP), insulin-like growth factor I (IGF-I), and interleukin-6 (IL-6). Neither loading (Rest leg vs. EX leg) nor treatment (control vs. ERT) influenced peritendinous PINP, whereas combined exercise and ERT enhanced muscle PINP after 72 h (interaction between loading and treatment P = 0.008). In neither skeletal muscle nor peritendinous fluid were IGF-I and IL-6 influenced by treatment or exercise. In conclusion, ERT was associated with enhanced synthesis of type I collagen in the skeletal muscle in response to acute exercise. In perspective, this indicates that the availability of estrogen in postmenopausal women is important for repair of muscle damage or remodeling of the connective tissue within the skeletal muscle after exercise.
绝经与皮肤和肌腱中胶原蛋白含量的减少以及骨骼肌中非收缩组织的积累有关。激素和体育活动对这些变化的相对作用尚不清楚。因此，我们在一项随机、对照、交叉研究中，调查了经皮雌激素替代疗法（ERT）对 11 名绝经后妇女肌腱和骨骼肌中 I 型胶原合成的影响。将雌激素贴片（Evorel）贴在髌腱上方的皮肤上，并与无贴片（对照期）进行比较。第 2 天，所有受试者进行单腿运动，然后比较运动腿（EX 腿）和非运动腿（休息腿）。在第 3 天和第 5 天，在髌腱前和两侧股外侧肌放置微透析导管。收集的透析液用于分析前胶原 I 型 NH（2）-末端前肽（PINP）、胰岛素样生长因子 I（IGF-I）和白细胞介素-6（IL-6）。无论是负荷（休息腿与 EX 腿）还是治疗（对照与 ERT）都不会影响腱周 PINP，而联合运动和 ERT 可增强 72 小时后的肌肉 PINP（负荷和治疗之间的相互作用 P = 0.008）。在骨骼肌和腱周液中，IGF-I 和 IL-6 均不受治疗或运动的影响。总之，ERT 与急性运动后骨骼肌 I 型胶原合成的增强有关。从长远来看，这表明绝经后妇女体内雌激素的存在对于运动后肌肉损伤的修复或骨骼肌内结缔组织的重塑是重要的。
