Li H, Zhang Y, Ning Z H, Deng X M, Lian Z X, Li N
State Key Laboratory of Agrobiotechnology, China Agricultural University, Beijing, PR China.
Poult Sci. 2008 Jan;87(1):41-9. doi: 10.3382/ps.2007-00306.
In current study, phagocytosis product (PP) of peripheral blood monocytes was detected among 920 dwarf chickens (460 per sex) at 20 wk of age, and based on discrepancies of PP, the flock was grouped (the highest group, the medium group, and the lowest group). Then serum hemagglutination inhibition antibody titers and subpopulations of T-lymphocytes of each group were examined after inoculations of avian influenza virus H5N2 inactivated vaccine (20 wk of age), avian influenza virus H9 inactivated vaccine (24 wk of age), and Newcastle disease virus-egg drop syndrome virus bigeminal inactivated vaccine (28 wk of age), respectively, to study the relationship between PP and immune response. To gain insight into effects of selection for PP on number of eggs, mean egg weight, fertilization rate, hatchability, and rate of healthy chicks, 9 (3 x 3) mating combinations were conducted. The results showed that (1) selection for higher PP in both sexes benefited to humoral immunity but not CD8(+) T-lymphocyte mediated immunity in dwarf chickens; (2) there were effects of selection for higher PP in hens on fertilization rate (P < 0.05), hatchability (P < 0.05), rate of healthy chicks (P < 0.05), and level of IgY antibody (P < 0.0001); however, hens' PP had no effects on number of eggs (P > or = 0.05) or egg weight (P > or = 0.05) and cocks' PP had no effect (P > or = 0.05) on any trait mentioned above. The results indicated that phagocytosis of peripheral blood monocytes might be an indicator of humoral immunity in dwarf chickens; furthermore, selection of hens with higher PP was not only beneficial to fertilization rate, but also benefited to hatchability and rate of healthy chicks in that the hens had stronger humoral immunity, which might contribute to maternal antibody in eggs.
在当前研究中，对920只20周龄矮小型鸡（雌雄各460只）外周血单核细胞的吞噬产物（PP）进行了检测，并根据PP的差异将鸡群分组（最高组、中等组和最低组）。然后，分别在接种禽流感病毒H5N2灭活疫苗（20周龄）、禽流感病毒H9灭活疫苗（24周龄）和新城疫病毒-减蛋综合征病毒二联灭活疫苗（28周龄）后，检测每组的血清血凝抑制抗体效价和T淋巴细胞亚群，以研究PP与免疫反应之间的关系。为了深入了解选择PP对产蛋数、平均蛋重、受精率、孵化率和健雏率的影响，进行了9种（3×3）交配组合。结果表明：（1）在矮小型鸡中，选择两性中PP较高的个体有利于体液免疫，但对CD8(+) T淋巴细胞介导的免疫无益处；（2）选择母鸡中PP较高的个体对受精率（P<0.05）、孵化率（P<0.05）、健雏率（P<0.05）和IgY抗体水平（P<0.0001）有影响；然而，母鸡的PP对产蛋数（P≥0.05）或蛋重（P≥0.05）无影响，公鸡的PP对上述任何性状均无影响（P≥0.05）。结果表明，外周血单核细胞的吞噬作用可能是矮小型鸡体液免疫的一个指标；此外，选择PP较高的母鸡不仅有利于提高受精率，还有利于提高孵化率和健雏率，因为这些母鸡具有较强的体液免疫，这可能有助于蛋中的母源抗体。
