Zlotnik Alexander, Gurevich Boris, Cherniavsky Evgenia, Tkachov Sergei, Matuzani-Ruban Angela, Leon Avner, Shapira Yoram, Teichberg Vivian I
Division of Anesthesiology and Critical Care, Soroka Medical Center, Ben-Gurion University of the Negev, Beer-Sheva, Israel.
Neurochem Res. 2008 Jun;33(6):1044-50. doi: 10.1007/s11064-007-9548-x. Epub 2007 Dec 14.
The removal of excess glutamate from brain fluids after acute insults such as closed head injury (CHI) and stroke is expected to prevent excitotoxicity and the ensuing long lasting neurological deficits. Since blood glutamate scavenging accelerates the removal of excess glutamate from brain into blood and causes neuroprotection, we have evaluated here whether the neuroprotective properties of pyruvate could be partly accounted to its blood glutamate scavenging activity. The neurological outcome of rats after CHI improved significantly when treated with intravenous pyruvate (0.9 mmoles/100 g) but not with pyruvate administered together with glutamate. Pyruvate, at 5 micromole/100 g rat was neither protective not able to decrease blood glutamate but displayed the latter two properties when combined with 60 microg/100 g of glutamate-pyruvate transaminase. Since the neurological recovery from CHI was correlated with the decrease of blood glutamate levels, we conclude that pyruvate blood glutamate scavenging activity contributes to the spectrum of its neuroprotective mechanisms.
在诸如闭合性颅脑损伤（CHI）和中风等急性损伤后，从脑液中清除过量谷氨酸有望预防兴奋性毒性及随之而来的长期神经功能缺损。由于血液谷氨酸清除可加速过量谷氨酸从脑内进入血液并产生神经保护作用，我们在此评估了丙酮酸的神经保护特性是否部分归因于其血液谷氨酸清除活性。CHI后大鼠经静脉注射丙酮酸（0.9毫摩尔/100克）治疗时神经功能结局显著改善，但与谷氨酸一起给药时则不然。5微摩尔/100克大鼠剂量的丙酮酸既无保护作用也不能降低血液谷氨酸水平，但与60微克/100克的谷氨酸 - 丙酮酸转氨酶联合使用时则表现出后两种特性。由于CHI后的神经功能恢复与血液谷氨酸水平的降低相关，我们得出结论，丙酮酸的血液谷氨酸清除活性有助于其神经保护机制的范围。
