Xu Chenjie, Xie Jin, Kohler Nathan, Walsh Edward G, Chin Y Eugene, Sun Shouheng
Department of Chemistry, Brown University, Providence, Rhode Island 02912, USA, Fax: (+1) 401-863-9046.
Brown Medical School, Brown University, Providence, Rhode Island 02906, USA.
Chem Asian J. 2008 Mar 7;3(3):548-552. doi: 10.1002/asia.200700301.
Functionalization of monodisperse superparamagnetic magnetite (Fe(3)O(4)) nanoparticles for cell specific targeting is crucial for cancer diagnostics and therapeutics. Targeted magnetic nanoparticles can be used to enhance the tissue contrast in magnetic resonance imaging (MRI), to improve the efficiency in anticancer drug delivery, and to eliminate tumor cells by magnetic fluid hyperthermia. Herein we report the nucleus-targeting Fe(3)O(4) nanoparticles functionalized with protein and nuclear localization signal (NLS) peptide. These NLS-coated nanoparticles were introduced into the HeLa cell cytoplasm and nucleus, where the particles were monodispersed and non-aggregated. The success of labeling was examined and identified by fluorescence microscopy and MRI. The work demonstrates that monodisperse magnetic nanoparticles can be readily functionalized and stabilized for potential diagnostic and therapeutic applications.
单分散超顺磁性磁铁矿（Fe(3)O(4)）纳米颗粒的功能化用于细胞特异性靶向，对癌症诊断和治疗至关重要。靶向磁性纳米颗粒可用于增强磁共振成像（MRI）中的组织对比度，提高抗癌药物递送效率，并通过磁流体热疗消除肿瘤细胞。在此，我们报告了用蛋白质和核定位信号（NLS）肽功能化的细胞核靶向Fe(3)O(4)纳米颗粒。这些涂有NLS的纳米颗粒被引入HeLa细胞的细胞质和细胞核中，颗粒在其中呈单分散且不聚集。通过荧光显微镜和MRI检查并鉴定了标记的成功情况。这项工作表明，单分散磁性纳米颗粒可以很容易地进行功能化和稳定化，以用于潜在的诊断和治疗应用。
