Gao Huajian, Shi Wendong, Freund Lambert B
Max Planck Institute for Metals Research, Heisenbergstrasse 3, D-70569 Stuttgart, Germany.
Proc Natl Acad Sci U S A. 2005 Jul 5;102(27):9469-74. doi: 10.1073/pnas.0503879102. Epub 2005 Jun 22.
Most viruses and bioparticles endocytosed by cells have characteristic sizes in the range of tens to hundreds of nanometers. The process of viruses entering and leaving animal cells is mediated by the binding interaction between ligand molecules on the viral capid and their receptor molecules on the cell membrane. How does the size of a bioparticle affect receptor-mediated endocytosis? Here, we study how a cell membrane containing diffusive mobile receptors wraps around a ligand-coated cylindrical or spherical particle. It is shown that particles in the size range of tens to hundreds of nanometers can enter or exit cells via wrapping even in the absence of clathrin or caveolin coats, and an optimal particles size exists for the smallest wrapping time. This model can also be extended to include the effect of clathrin coat. The results seem to show broad agreement with experimental observations.
大多数被细胞内吞的病毒和生物颗粒具有数十到数百纳米范围内的特征尺寸。病毒进出动物细胞的过程是由病毒衣壳上的配体分子与其细胞膜上的受体分子之间的结合相互作用介导的。生物颗粒的大小如何影响受体介导的内吞作用？在这里，我们研究含有扩散性移动受体的细胞膜如何包裹在配体包被的圆柱形或球形颗粒周围。结果表明，即使在没有网格蛋白或小窝蛋白包被的情况下，数十到数百纳米尺寸范围内的颗粒也可以通过包裹进入或离开细胞，并且存在一个使包裹时间最短的最佳颗粒尺寸。该模型还可以扩展到包括网格蛋白包被的影响。结果似乎与实验观察结果广泛一致。
