Abu Al-Soud W, Stenram U, Ljungh A, Tranberg K-G, Nilsson H-O, Wadström T
Section of Medical Microbiology, Department of Laboratory Medicine, Lund University, Sölvegatan 23, SE-223 62 Lund, Sweden.
Dig Liver Dis. 2008 Feb;40(2):126-31. doi: 10.1016/j.dld.2007.09.011. Epub 2007 Dec 20.
Gastric and enteric Helicobacter species have been associated with the pathogenesis of some extragastric diseases.
We retrospectively investigated the presence of DNA of Helicobacter species in samples of the cancer and the surrounding tumour-free liver tissues of patients with hepatocellular carcinoma (HCC, n=12) and cholangiocarcinoma (CC, n=13). The patients were from an area with low liver cancer incidence and with low hepatitis B and C prevalence. Patients with a benign liver disease (n=24) were included as controls. Paraffin-embedded liver samples were examined by a Helicobacter genus-specific PCR assay as well as group-specific PCR assays for Enterobacteriaceae, Bacteroides, Lactobacillus and Enterococcus. PCR products of positive samples were characterised by denaturing gradient gel electrophoresis (DGGE) and DNA sequencing.
PCR assay detected Helicobacter DNA in seven of 12 (58%) and eight of 13 (62%) normal liver tissue specimens from HCC and CC patients, respectively. Two cancer samples from HCC patients were Helicobacter-positive but none of the CC cancers. In the control group, three of 24 (12.5%) patients with a benign liver condition were positive for Helicobacter species (p<0.01 compared to results of tumour-free liver tissue from the cancer patients). DGGE and DNA sequence analysis showed that 90% of the detected PCR products were "H. pylori-like". DNA of some other enteric bacteria was detected in the liver of one cancer patient and one control (4% of all patients).
The presence of DNA of Helicobacter species in liver specimens, but not of other common gut bacteria, was associated with human hepatic carcinogenesis.
胃内和肠道内的幽门螺杆菌属菌种已被证实与某些胃外疾病的发病机制有关。
我们回顾性调查了肝细胞癌（HCC，n = 12）和胆管癌（CC，n = 13）患者的癌组织样本及周围无肿瘤的肝组织样本中幽门螺杆菌属菌种的DNA存在情况。这些患者来自肝癌发病率低且乙肝和丙肝患病率低的地区。纳入24例患有良性肝病的患者作为对照。采用幽门螺杆菌属特异性PCR检测法以及针对肠杆菌科、拟杆菌属、乳酸杆菌属和肠球菌属的组特异性PCR检测法对石蜡包埋的肝脏样本进行检测。对阳性样本的PCR产物进行变性梯度凝胶电泳（DGGE）分析和DNA测序。
PCR检测分别在12例HCC患者的7例（58%）和13例CC患者的8例（62%）正常肝组织标本中检测到幽门螺杆菌DNA。2例HCC患者的癌组织样本呈幽门螺杆菌阳性，但CC癌组织样本均为阴性。在对照组中，24例患有良性肝病的患者中有3例（12.5%）幽门螺杆菌属菌种呈阳性（与癌症患者的无肿瘤肝组织结果相比，p < 0.01）。DGGE和DNA序列分析表明，90%的检测到的PCR产物为“幽门螺杆菌样”。在1例癌症患者和1例对照的肝脏中检测到一些其他肠道细菌的DNA（占所有患者的4%）。
肝脏标本中存在幽门螺杆菌属菌种的DNA，而非其他常见肠道细菌的DNA，与人类肝癌发生有关。
