Tropical Disease Research Laboratory, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand.
Department of Pathology, Faculty of Medicine, Khon Kaen University, Khon Kaen, 40002, Thailand.
Sci Rep. 2017 Feb 15;7:42744. doi: 10.1038/srep42744.
Recent reports suggest that Opisthorchis viverrini serves as a reservoir of Helicobacter and implicate Helicobacter in pathogenesis of opisthorchiasis-associated cholangiocarcinoma (CCA). Here, 553 age-sex matched cases and controls, 293 and 260 positive and negative for liver fluke O. viverrini eggs, of residents in Northeastern Thailand were investigated for associations among infection with liver fluke, Helicobacter and hepatobiliary fibrosis. The prevalence of H. pylori infection was higher in O. viverrini-infected than uninfected participants. H. pylori bacterial load correlated positively with intensity of O. viverrini infection, and participants with opisthorchiasis exhibited higher frequency of virulent cagA-positive H. pylori than those free of fluke infection. Genotyping of cagA from feces of both infected and uninfected participants revealed that the AB genotype accounted for 78% and Western type 22%. Participants infected with O. viverrini exhibited higher prevalence of typical Western type (EPIYA ABC) and variant AB'C type (EPIYT B) CagA. Multivariate analyses among H. pylori virulence genes and severity of hepatobiliary disease revealed positive correlations between biliary periductal fibrosis during opisthorchiasis and CagA and CagA with CagA multimerization (CM) sequence-positive H. pylori. These findings support the hypothesis that H. pylori contributes to the pathogenesis of chronic opisthorchiasis and specifically to opisthorchiasis-associated CCA.
最近的报告表明，肝片形吸虫是幽门螺杆菌的储存宿主，并暗示幽门螺杆菌在肝片形吸虫相关胆管癌（CCA）的发病机制中起作用。在这里，对泰国东北部的 553 名年龄和性别匹配的病例和对照者（293 名肝吸虫卵阳性，260 名肝吸虫卵阴性）进行了研究，以调查肝吸虫感染、幽门螺杆菌感染和肝胆纤维化之间的关系。在肝吸虫感染参与者中，幽门螺杆菌感染的患病率高于未感染参与者。幽门螺杆菌细菌负荷与肝片形吸虫感染强度呈正相关，且患有肝片形吸虫病的参与者中，毒力 cagA 阳性幽门螺杆菌的频率高于未感染肝吸虫的参与者。对感染和未感染参与者粪便中的 cagA 进行基因分型显示，AB 基因型占 78%，西方 2 型占 22%。感染肝片形吸虫的参与者表现出更高的典型西方型（EPIYA ABC）和变异 AB'C 型（EPIYT B）CagA 的患病率。在幽门螺杆菌毒力基因和肝胆疾病严重程度之间的多变量分析中，发现在肝片形吸虫感染期间胆管周围纤维化与 CagA 呈正相关，CagA 与 CagA 多聚体化（CM）序列阳性的幽门螺杆菌呈正相关。这些发现支持了这样一种假设，即幽门螺杆菌有助于慢性肝片形吸虫病的发病机制，特别是与肝片形吸虫相关的 CCA。
