Araújo Núbia S, Mundim Maria J S, Gomes Maria A, Amorim Rúbia M R, Viana João C, Queiroz Rodrigo P, Rossi Marcos A, Cury Márcia C
Laboratory of Parasitology, Institute of Biomedical Sciences, Federal University of Uberlândia, Av. Pará n(o)1720 Bloco 4C Campus Umuarama, 38400-902, Uberlândia, MG, Brazil.
Exp Parasitol. 2008 Apr;118(4):449-57. doi: 10.1016/j.exppara.2007.10.007. Epub 2007 Oct 13.
To examine the infection kinetics and development of alterations in the small intestine of gerbils (Meriones unguiculatus), 72 gerbils were divided into six groups (A to F), with A serving as control and the others inoculated with increasing doses of trophozoites from Giardia duodenalis human isolate. The infection kinetics and the development of histopathological alterations were monitored by optical scanning electron microscopy (SEM). A 12-day prepatent period was observed, with intermittent elimination up to day 35 after inoculation. Statistically significant differences were found between the mean number of trophozoites recovered, per group, on the days of sacrifice, and a positive correlation between the inoculum dosage and the number of trophozoites recovered. Morphometrically, the villus:crypt ratio showed a drop in all the groups when compared with the control group. SEM revealed an increase in mucus production in the inoculated animals and the presence of trophozoite clusters at the top and base of the villi. The dosage of trophozoite inoculum does not interfere in the ability for infection to occur or in the development of histopathological alterations generated by intestinal colonization.
为研究沙土鼠（长爪沙鼠）小肠感染动力学及变化发展情况，将72只沙土鼠分为六组（A至F组），A组作为对照组，其他组接种来自人十二指肠贾第鞭毛虫分离株的递增剂量滋养体。通过光学扫描电子显微镜（SEM）监测感染动力学及组织病理学变化发展情况。观察到潜伏期为12天，接种后至第35天滋养体间歇性排出。在处死日每组回收的滋养体平均数量之间发现有统计学显著差异，接种剂量与回收的滋养体数量呈正相关。形态学上，与对照组相比，所有组的绒毛：隐窝比值均下降。扫描电子显微镜显示接种动物的黏液分泌增加，且在绒毛顶部和底部存在滋养体聚集。滋养体接种剂量不影响感染发生的能力或肠道定植产生的组织病理学变化的发展。
