采用酶联免疫吸附测定法（ELISA）对日本梅花鹿（Cervus nippon yesoensis）进行莱姆病血清学调查。

Serological survey for Lyme disease in sika deer (Cervus nippon yesoensis) by enzyme-linked immunosorbent assay (ELISA).

作者信息

Isogai E, Isogai H, Masuzawa T, Yanagihara Y, Sato N, Hayashi S, Maki T, Mori M

机构信息

Department of Preventative Dentistry, Higashi Nippon Gakuen University, Hokkaido, Japan.

出版信息

Microbiol Immunol. 1991;35(9):695-703. doi: 10.1111/j.1348-0421.1991.tb01603.x.

DOI:10.1111/j.1348-0421.1991.tb01603.x

PMID:1808467

Abstract

Seventy-six wild sika deer (Cervus nippon yesoensis) from areas endemic for Borrelia burgdorferi during 1988 to 1989 had the IgG antibody to the bacteria in enzyme-linked immunosorbent assay. The percentage of seropositive deer was 25.0% to strain HO14 and 22.4% to strain HP3, respectively. Specific IgG antibody titers were high in summer but low in winter. In summer, seropositive deer were 75.0%. Similar results were obtained in serum specimens obtained monthly from 4 farmed deer. It was suggested that the sika deer could be one of the wild reservoirs for B. burgdorferi in Hokkaido, Japan. The transmission risk of B. burgdorferi is considered to be greatest during late spring to early summer.

摘要

1988年至1989年期间，从莱姆病（Borrelia burgdorferi）流行地区采集了76只野生梅花鹿（Cervus nippon yesoensis），通过酶联免疫吸附测定法检测其针对该细菌的IgG抗体。血清阳性鹿针对HO14菌株的比例为25.0%，针对HP3菌株的比例为22.4%。特异性IgG抗体滴度夏季高而冬季低。夏季血清阳性鹿为75.0%。从4只养殖梅花鹿每月采集的血清标本也得到了类似结果。提示梅花鹿可能是日本北海道莱姆病螺旋体的野生宿主之一。莱姆病螺旋体的传播风险在晚春至初夏期间被认为最大。