通过聚合酶链反应（PCR）检测自然感染的野生梅花鹿（日本北海道梅花鹿）皮肤中的莱姆病螺旋体。
Detection of Lyme disease spirochetes in the skin of naturally infected wild sika deer (Cervus nippon yesoensis) by PCR.
作者信息
Kimura K, Isogai E, Isogai H, Kamewaka Y, Nishikawa T, Ishii N, Fujii N
机构信息
Department of Microbiology, Sapporo Medical University, Japan.
出版信息
Appl Environ Microbiol. 1995 Apr;61(4):1641-2. doi: 10.1128/aem.61.4.1641-1642.1995.
Abstract
We demonstrated the presence of Borrelia burgdorferi sensu lato DNA in the skin tissues of naturally infected wild sika deer, using PCR. The risk of transmission of B. burgdorferi sensu lato is recognized in sika deer.
摘要
我们通过聚合酶链反应（PCR）证明了在自然感染的野生梅花鹿皮肤组织中存在伯氏疏螺旋体狭义种DNA。梅花鹿存在传播狭义伯氏疏螺旋体的风险。
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本文引用的文献
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Experimental Borrelia garinii infection of Japanese quail.日本鹌鹑的实验性莱姆病螺旋体感染。
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Lyme disease-a tick-borne spirochetosis?莱姆病——一种蜱传播的螺旋体病？
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Avian and mammalian hosts for spirochete-infected ticks and insects in a Lyme disease focus in Connecticut.在康涅狄格州的一个莱姆病疫源地，感染螺旋体的蜱虫和昆虫的鸟类及哺乳动物宿主。
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Reduced abundance of immature Ixodes dammini (Acari: Ixodidae) following elimination of deer.鹿被清除后未成熟的达氏硬蜱（蜱螨亚纲：硬蜱科）数量减少。
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Lyme borreliosis: relation of its causative agent to its vectors and hosts in North America and Europe.莱姆病：其病原体在北美和欧洲与其传播媒介及宿主的关系
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Serological survey for Lyme disease in sika deer (Cervus nippon yesoensis) by enzyme-linked immunosorbent assay (ELISA).采用酶联免疫吸附测定法（ELISA）对日本梅花鹿（Cervus nippon yesoensis）进行莱姆病血清学调查。
Microbiol Immunol. 1991;35(9):695-703. doi: 10.1111/j.1348-0421.1991.tb01603.x.
10
Polymerase chain reaction primers and probes derived from flagellin gene sequences for specific detection of the agents of Lyme disease and North American relapsing fever.源自鞭毛蛋白基因序列的聚合酶链反应引物和探针，用于莱姆病和北美回归热病原体的特异性检测。
J Clin Microbiol. 1992 Jan;30(1):99-114. doi: 10.1128/jcm.30.1.99-114.1992.
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