将一种商用酶联免疫吸附测定法（ELISA）与间接荧光抗体试验进行比较，以检测经实验性攻毒的猪体内抗胞内劳森菌的抗体。
Comparison of a commercial ELISA with an indirect fluorescent antibody test to detect antibodies to Lawsonia intracellularis in experimentally challenged pigs.
作者信息
Collins A, Gonsalves J, Fell S, Barchia I
机构信息
Department of Primary Industries New South Wales, Elizabeth Macarthur Agricultural Institute, Narellan, New South Wales, Australia.
出版信息
Aust Vet J. 2012 Mar;90(3):97-9. doi: 10.1111/j.1751-0813.2011.00865.x.
OBJECTIVE
The ability of a new commercial ELISA to detect pigs with subclinical proliferative enteropathy (PE) was compared with the traditional indirect fluorescent antibody test (IFAT).
METHODS
Serum samples were selected from pigs with known Lawsonia intracellularis infection status and clinical signs of PE, but the sample population consisted predominantly of pigs subclinically affected by PE.
RESULTS
Significant association and agreement were shown between the ELISA and IFAT assays. ELISA results correlated well with the duration of L. intracellularis shedding as detected by polymerase chain reaction.
CONCLUSION
ELISA can be successfully used to monitor L. intracellularis infection in pigs.
目的
将一种新型商用酶联免疫吸附测定（ELISA）检测亚临床增生性肠炎（PE）猪的能力与传统间接荧光抗体试验（IFAT）进行比较。
方法
血清样本选自已知感染胞内劳森菌且有PE临床症状的猪，但样本群体主要是受PE亚临床影响的猪。
结果
ELISA和IFAT检测之间显示出显著的相关性和一致性。ELISA结果与通过聚合酶链反应检测到的胞内劳森菌 shedding 持续时间密切相关。
结论
ELISA可成功用于监测猪的胞内劳森菌感染。
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