Mendez S, D' Samuel A, Antoine A D, Ahn S, Hotez P
Department of Microbiology, Immunology and Tropical Medicine, The George Washington University Medical Center and The Sabin Vaccine Institute, Washington DC, USA.
Parasite Immunol. 2008 Jan;30(1):53-6. doi: 10.1111/j.1365-3024.2007.00994.x.
Hookworms are gastrointestinal nematodes that affect approximately 600 million people in developing countries. Using the air pouch model, we have examined the effects of vaccination with the recombinant hookworm larval antigen Na-ASP-2 and the adjuvant Alhydrogel on the skin immune response to hookworms in Sprague Dawley rats. Following vaccination, rats were inoculated 100 Necator americanus L3 into the air pouch, and the exudates and cell infiltrates were collected from the pouch 24 h later. Larval inoculation induced leucocyte recruitment into the pouch. Exudates of rats vaccinated with Na-ASP-2 showed an increase of cytokines such as IL-4, IL-10, IFN-gamma and especially, IL-5, as well as IgG1 and IgG2 antibodies. The increased amount of antigen-presenting cells and cytokines in the pouch of vaccinated animals suggests that vaccination could potentially restrain this parasite to the inoculation site, avoiding its migration and establishment in the host. Moreover, the air pouch model could constitute an alternative to screen immune responses to L3 antigens.
钩虫是一种胃肠道线虫，在发展中国家约有6亿人受其影响。我们使用气袋模型，研究了用重组钩虫幼虫抗原Na-ASP-2和佐剂氢氧化铝凝胶进行疫苗接种对斯普拉格-道利大鼠皮肤针对钩虫的免疫反应的影响。疫苗接种后，将100条美洲板口线虫L3接种到大鼠的气袋中，24小时后从气袋中收集渗出液和细胞浸润物。幼虫接种诱导白细胞募集到气袋中。用Na-ASP-2接种的大鼠的渗出液显示细胞因子如IL-4、IL-10、IFN-γ尤其是IL-5以及IgG1和IgG2抗体增加。接种疫苗动物气袋中抗原呈递细胞和细胞因子数量的增加表明，疫苗接种可能会将这种寄生虫限制在接种部位，避免其在宿主体内迁移和定植。此外，气袋模型可作为筛选针对L3抗原的免疫反应的一种替代方法。
