Iyer Rishyashring R, Žurauskas Mantas, Cui Qi, Gao Liang, Theodore Smith R, Boppart Stephen A
Beckman Institute for Advanced Science and Technology, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA.
Department of Electrical and Computer Engineering, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA.
Biomed Opt Express. 2020 Sep 28;11(10):5903-5919. doi: 10.1364/BOE.402796. eCollection 2020 Oct 1.
Prevalent techniques in label-free linear optical microscopy are either confined to imaging in two dimensions or rely on scanning, both of which restrict their applications in imaging subtle biological dynamics. In this paper, we present the theoretical basis along with demonstrations supporting that full-field spectral-domain interferometry can be used for imaging samples in 3D with no moving parts in a single shot. Consequently, we propose a novel optical imaging modality that combines low-coherence interferometry with hyperspectral imaging using a light-emitting diode and an image mapping spectrometer, called Snapshot optical coherence microscopy (OCM). Having first proved the feasibility of Snapshot OCM through theoretical modeling and a comprehensive simulation, we demonstrate an implementation of the technique using off-the-shelf components capable of capturing an entire volume in 5 ms. The performance of Snapshot OCM, when imaging optical targets, shows its capability to axially localize and section images over an axial range of ±10 µm, while maintaining a transverse resolution of 0.8 µm, an axial resolution of 1.4 µm, and a sensitivity of up to 80 dB. Additionally, its performance in imaging weakly scattering live cells shows its capability to not only localize the cells in a densely populated culture but also to generate detailed phase profiles of the structures at each depth for long durations. Consolidating the advantages of several widespread optical microscopy modalities, Snapshot OCM has the potential to be a versatile imaging technique for a broad range of applications.
无标记线性光学显微镜中的现有技术要么局限于二维成像，要么依赖扫描，这两者都限制了它们在成像细微生物动态方面的应用。在本文中，我们阐述了理论基础，并通过演示证明了全场光谱域干涉测量法可用于单次对三维样本成像且无需移动部件。因此，我们提出了一种新型光学成像模式，即结合低相干干涉测量法与使用发光二极管和图像映射光谱仪的高光谱成像，称为快照光学相干显微镜（OCM）。我们首先通过理论建模和全面模拟证明了快照OCM的可行性，然后展示了使用现成组件对该技术的实现，这些组件能够在5毫秒内捕获整个体积。快照OCM在对光学目标成像时的性能表明，它能够在±10 µm的轴向范围内进行轴向定位和切片成像，同时保持横向分辨率为0.8 µm、轴向分辨率为1.4 µm以及高达80 dB的灵敏度。此外，它在对弱散射活细胞成像时的性能表明，它不仅能够在密集培养物中定位细胞，还能够长时间生成每个深度处结构的详细相位分布图。整合了几种广泛应用的光学显微镜模式的优点，快照OCM有潜力成为一种适用于广泛应用的通用成像技术。
