Smits G A, Heerschap A, Oosterhof G O, Ruys J H, Hilbers C W, Debruyne F M, Schalken J A
Department of Urology and Radiology, St. Radboud University Hospital, Nijmegen, The Netherlands.
Ultrasound Med Biol. 1991;17(8):791-801. doi: 10.1016/0301-5629(91)90162-p.
The effects of electromagnetically generated high-energy shock waves (HESW, Siemens Lithostar) on the phosphate metabolite levels of the NU-1 human kidney cancer xenograft implanted under the skin of the hind limb of nude mice were monitored by 31P magnetic resonance spectroscopy (MRS). Administration of 200 and 800 HESW (18.1 kV, P(+) = 37.5 MPa, P(-) = 5.2 MPa, tr = 30-120 nsec, tw = 340 nsec, freq. = 1.25 Hz), focused on the tumor centre, resulted in an immediate tumor decline; 2 h after exposure to the HESW, the high-energy phosphate resonances had decreased drastically. This decline in energy rich molecules was accompanied by a concomitant increase in the inorganic phosphate resonance and a decrease in pH of the tumor. During the following period, a dose-time dependent recovery of the original high-energy phosphate resonance intensities was observed. These changes are qualitatively similar to those produced by ischemic inhibition of energy metabolism and are correlated with early histological features like vascular disruption, stasis within capillaries, and focal thrombosis. These results demonstrate that experimental HESW treatment of the NU-1 kidney tumor is effective in provoking a temporary reduction of both high-energy phosphate metabolism and tissue pH of the tumor. The data presented here strongly suggest that these effects are predominantly indirect by affecting tumor vascularity. Overall, this study shows that MRS is a powerful technique for longitudinal investigations of HESW-induced effects and can provide information about its mode of action.
通过31P磁共振波谱（MRS）监测电磁产生的高能冲击波（HESW，西门子Lithostar）对裸鼠后肢皮下植入的NU-1人肾癌异种移植物磷酸代谢物水平的影响。聚焦于肿瘤中心给予200次和800次HESW（18.1 kV，P(+) = 37.5 MPa，P(-) = 5.2 MPa，tr = 30 - 120纳秒，tw = 340纳秒，频率 = 1.25赫兹），导致肿瘤立即缩小；暴露于HESW后2小时，高能磷酸共振显著下降。富含能量分子的这种下降伴随着无机磷酸共振的相应增加以及肿瘤pH值的降低。在随后的时期，观察到原始高能磷酸共振强度呈剂量 - 时间依赖性恢复。这些变化在质量上与缺血性能量代谢抑制所产生的变化相似，并且与早期组织学特征如血管破坏、毛细血管内血流停滞和局灶性血栓形成相关。这些结果表明，对NU-1肾肿瘤进行实验性HESW治疗可有效引起肿瘤高能磷酸代谢和组织pH值的暂时降低。此处呈现的数据强烈表明，这些效应主要是通过影响肿瘤血管间接产生的。总体而言，本研究表明MRS是用于纵向研究HESW诱导效应的强大技术，并且可以提供有关其作用方式的信息。
