Smits G A, Cornel E B, van de Boogert E, Oosterhof G O, Debruyne F M, Schalken J A, Heerschap A
Department of Urology, University Hospital Nijmegen, The Netherlands.
NMR Biomed. 1994 Nov;7(7):319-26. doi: 10.1002/nbm.1940070705.
The effects of high energy shock waves (HESW) on tumor cell metabolism and tumor blood flow were studied in the NU-1 kidney cancer xenograft by multinuclear 1H/2H/31P magnetic resonance spectroscopy. Tumor xenografts were exposed to 800 HESW using an experimental electromagnetic shock wave emitter based on the Siemens Lithostar Plus, which is used for clinical lithotripsy. Exposure of tumors to 800 HESW resulted in a temporary decrease of tumor blood flow (TBF) determined by the 2H NMR monitoring of the 2HO1H wash-out after intratumoral injection. By concomitant recording of 31P and 1H NMR spectra, tumor pH, high-energy phosphates and lactate levels were followed. Tumor treatment with HESW transiently resulted in acidification, ATP decrease, P(i) increase and lactate increase. In contrast, HESW administration adjacent to the tumor did not significantly influence TBF, tumor pH, high-energy phosphates or lactate levels, showing that the observed alterations are caused by an interaction of HESW and tumor tissue. The most likely explanation for these observations is that HESW administration causes local vascular malfunctioning followed by a reduction in oxygen and nutrient supply to the tumor which leads to a decreased aerobic energy metabolism. The results of this study may be used to aid the design of HESW-based therapies.
利用多核1H/2H/31P磁共振波谱技术，在NU-1肾癌异种移植模型中研究了高能冲击波（HESW）对肿瘤细胞代谢和肿瘤血流的影响。使用基于西门子Lithostar Plus的实验性电磁冲击波发射器，将肿瘤异种移植体暴露于800次HESW下，该发射器用于临床碎石术。通过肿瘤内注射后对2HO1H洗脱的2H NMR监测，发现肿瘤暴露于800次HESW后，肿瘤血流（TBF）暂时下降。通过同时记录31P和1H NMR谱，跟踪肿瘤pH值、高能磷酸盐和乳酸水平。HESW治疗肿瘤会导致短暂的酸化、ATP降低、P(i)升高和乳酸增加。相比之下，在肿瘤附近施加HESW对TBF、肿瘤pH值、高能磷酸盐或乳酸水平没有显著影响，表明观察到的变化是由HESW与肿瘤组织的相互作用引起的。对这些观察结果最可能的解释是，施加HESW会导致局部血管功能障碍，随后肿瘤的氧气和营养供应减少，从而导致有氧能量代谢降低。本研究结果可用于辅助基于HESW的治疗方法的设计。
