Cundell A M, Mueller W C, Traxler R W
Appl Environ Microbiol. 1976 Mar;31(3):408-14. doi: 10.1128/aem.31.3.408-414.1976.
The morphology and ultrastructure of a Penicillium sp. grown on n-hexadecane or on peptone in shake-culture were compared using transmission and scanning electron microscopy. The fungus grew as hollow mycelial balls surrounding individual hydrocarbon droplets on n-hexadecane and as solid mycelial balls on peptone. A dense layer of fungal mycelium that showed irregular forms, fusion, and increase in hyphal size formed at the hydrocarbon-water interface. Inclusions were present in the hexadecane-grown fungus that were absent when the Penicillium sp. was grown on peptone. Problems of fixation made it difficult to differentiate detailed changes in the cytoplasm when the fungus was examined with the transmission electron microscope.
利用透射电子显微镜和扫描电子显微镜，比较了在摇瓶培养中生长于正十六烷或蛋白胨上的青霉属真菌的形态和超微结构。该真菌在正十六烷上生长时形成围绕单个烃滴的空心菌丝球，而在蛋白胨上生长时形成实心菌丝球。在烃 - 水界面形成了一层致密的真菌菌丝体，其呈现出不规则形态、融合以及菌丝尺寸增加的现象。在正十六烷上生长的真菌中存在内含物，而该青霉属真菌在蛋白胨上生长时则不存在这些内含物。由于固定问题，在用透射电子显微镜检查真菌时，难以区分细胞质中的详细变化。
