Scott C C, Finnerty W R
J Bacteriol. 1976 Jul;127(1):481-9. doi: 10.1128/jb.127.1.481-489.1976.
The ultrastructure of Acinetobacter sp. strain HO1-N grown on hydrocarbon and nonhydrocarbon substrates was compared using thin sections and freeze-etching. Hydrocarbon-grown cells were characterized by the presence of intracytoplasmic membrane-bound hexadecane inclusions. This membrane did not exhibit a typical unit membrane structure but appeared as a monolayer. The freeze-etch technique revealed the internal structure of the hexadecane inclusions and provided evidence for the presence of a smooth-surfaced limiting membrane. Freeze-etching also revealed intracytoplasmic membranes in the hexadecane-grown cells. These ultrastructural modifications were not present in nonhydrocarbon-grown cells. The hexadecane inclusions were isolated from Acinetobacter. Negative-staining of the inclusions revealed electron-transparent vesicles approximating the size of the inclusions seen in whole cells. Freeze-etching of the purified inclusions revealed membrane-bound vesicles. The purified inclusions exhibited a relatively high value of lipid phosphorus to protein. The lipid composition and the electrophoretic banding pattern of the inclusions on sodium dodecyl sulfate-polyacrylamide gels were determined and compared with other membrane fractions (outer membrane and cytoplasmic membrane) previously isolated from this organism.
利用超薄切片和冷冻蚀刻技术，比较了不动杆菌属HO1-N菌株在烃类和非烃类底物上生长时的超微结构。以烃类为底物生长的细胞的特征是存在胞质内与膜结合的十六烷包涵体。这种膜不呈现典型的单位膜结构，而是呈现为单层。冷冻蚀刻技术揭示了十六烷包涵体的内部结构，并为存在光滑表面的限制膜提供了证据。冷冻蚀刻还揭示了在以十六烷为底物生长的细胞中的胞内膜。这些超微结构修饰在以非烃类为底物生长的细胞中不存在。从不动杆菌中分离出十六烷包涵体。对包涵体进行负染色显示出与全细胞中所见包涵体大小相近的电子透明囊泡。对纯化的包涵体进行冷冻蚀刻显示出与膜结合的囊泡。纯化的包涵体表现出相对较高的脂质磷与蛋白质比值。测定了包涵体的脂质组成以及在十二烷基硫酸钠-聚丙烯酰胺凝胶上的电泳条带模式，并与先前从该生物体中分离出的其他膜组分（外膜和细胞质膜）进行了比较。
