Reutgen H, Pokorny J, Christ R, Ritzau E
Forschungsinstitut für Lungenkrankheiten und Tuberkulose Berlin-Buch, BRD.
Z Erkr Atmungsorgane. 1991;177(1-2):103-10.
In connection with studies on the pathogenesis of sarcoidosis antigen fractions were isolated from 8 mycobacteria species, three out of each strain. These fractions were tested for their reactivity to serum antibodies by means of RIA-technique, using 40 selected sera from controls, and patients with sarcoidosis, tuberculosis and asthma. Comparing the results (average titer steps) sera from asthmatics showed the lowest and those from sarcoidosis patients the highest reactivities to the mycobacterial antigen fractions. The reactivities clearly differed in dependence on the mycobacteria species. The highest mean reactivity in sarcoidosis patients was found with the HIP-antigen fraction of M. xenopi. It was 8 times higher compared to the control sera as well as the tuberculosis sera and 32 times higher than that of the asthma sera. There were also clear differences in the reactivities within the sarcoidosis sera tested. In sera from patients with clinically inactive sarcoidosis there were found nearly the same or only slightly higher titer steps than in control sera as well as tuberculosis sera, however in clinically active sarcoidosis the titer steps were clearly elevated. The findings are seen in connection with the role of atypical mycobacteria (MOTT) in the pathogenesis of sarcoidosis. The potential applications of the HIP- and Triton X-100 antigen fractions for in vitro diagnostics are discussed.
在结节病发病机制的研究中，从8种分枝杆菌菌株中各分离出3种抗原组分。使用来自对照组、结节病患者、结核病患者和哮喘患者的40份选定血清，通过放射免疫分析技术检测这些组分与血清抗体的反应性。比较结果（平均滴度变化）发现，哮喘患者血清对分枝杆菌抗原组分的反应性最低，而结节病患者血清的反应性最高。反应性明显因分枝杆菌种类而异。在结节病患者中，对蟾分枝杆菌的HIP抗原组分反应性最高。与对照血清和结核病血清相比，其反应性高8倍，比哮喘血清高32倍。在所检测的结节病血清中，反应性也存在明显差异。在临床非活动性结节病患者的血清中，滴度变化与对照血清以及结核病血清几乎相同或仅略高，然而在临床活动性结节病中，滴度变化明显升高。这些发现与非典型分枝杆菌（MOTT）在结节病发病机制中的作用相关。讨论了HIP和Triton X - 100抗原组分在体外诊断中的潜在应用。
