Mazur L, Manowska J, Bobik R
Department of Animal Physiology, Jagiellonian University, Cracow, Poland.
Acta Physiol Hung. 1991;78(2):143-50.
Temporal changes of acid phosphatase (E.C. 3.1.3.2) and arylsulphatase (E.C. 3.1.6.1) activities in testes of adult Swiss mice after AET (2-amino-ethylisothiouronium Br. HBr) or MEA (cysteamine HCl) treatment, were studied. The animals were injected intraperitoneally with the S-containing substances in a single dose of 400 mg/kg body weight. The enzyme activities in crude organ homogenates were assessed every four hours during a 24-hour period. Administration of the aminothiol agents to mouse organism caused greater changes in the acid phosphatase activity than in the arylsulphatase activity, and the two chemical compounds AET and MEA given, influenced the enzyme activities in testes in a different way. Treatment of mice with AET resulted in a decrease of the acid phosphatase activity related to 1 g of fresh tissue at 16.00 and the whole organ weight at 24.00 and 16.00 as well as in a decrease of the arylsulphatase activity expressed per the whole weight of testes at 08.00. After MEA injection, the acid phosphatase activity related to 1 mg of protein, 1 g of fresh tissue and the whole organ weight was decreased at 20.00(1), and the enzyme activity expresse per 1 mg of protein and 1 g of fresh tissue was increased at 24.00, but the arylsulphatase activity related to both 1 mg of protein at 08.00, 12.00 and to the whole weight of testes at 08.00, was reduced.
研究了用AET（2-氨基乙基异硫脲溴化氢）或MEA（半胱胺盐酸盐）处理成年瑞士小鼠后，其睾丸中酸性磷酸酶（E.C. 3.1.3.2）和芳基硫酸酯酶（E.C. 3.1.6.1）活性的时间变化。给动物腹腔注射含硫物质，单剂量为400mg/kg体重。在24小时内，每4小时评估一次粗器官匀浆中的酶活性。给小鼠机体施用氨基硫醇试剂后，酸性磷酸酶活性的变化比芳基硫酸酯酶活性的变化更大，并且所给的两种化合物AET和MEA对睾丸中的酶活性有不同的影响。用AET处理小鼠导致16:00时每克新鲜组织的酸性磷酸酶活性降低，24:00和16:00时整个器官重量降低，以及08:00时以睾丸全重表示的芳基硫酸酯酶活性降低。注射MEA后，20:00时与1mg蛋白质、1g新鲜组织和整个器官重量相关的酸性磷酸酶活性降低，24:00时每1mg蛋白质和1g新鲜组织表示的酶活性增加，但08:00、12:00时与1mg蛋白质相关以及08:00时与睾丸全重相关的芳基硫酸酯酶活性降低。
