Mazur L, Bobik R, Manowska J
Department of Animal Physiology, Jagiellonian University, Cracow, Poland.
Acta Physiol Hung. 1994;82(4):405-14.
The adult male Swiss mice were treated intraperitoneally with AET (2-aminoethylisothiouronium Br.HBr) or MEA (cysteamine HCl), in a toxic dose of 400 mg/kg body weight. The measurements of the protein content in crude homogenates of testes, kidneys and liver were done every fourth hour throughout a 24-hour period. Treatment of mice with AET and MEA resulted in different patterns of temporal changes in the protein content in testes, kidneys and liver expressed in mg per 1 g of fresh tissue and per the whole organ weight. The extent and timing of the alterations in the protein level appeared to depend on the particular organ chosen and the thiol agent applied.
成年雄性瑞士小鼠腹腔注射给予AET（2-氨基乙基异硫脲溴化氢）或MEA（半胱胺盐酸盐），剂量为400mg/kg体重，此剂量具有毒性。在24小时内，每隔4小时对睾丸、肾脏和肝脏的粗匀浆中的蛋白质含量进行测量。用AET和MEA处理小鼠后，睾丸、肾脏和肝脏中蛋白质含量随时间变化的模式不同，以每1g新鲜组织和整个器官重量中的毫克数表示。蛋白质水平变化的程度和时间似乎取决于所选的特定器官和应用的硫醇试剂。
