Manowska J, Mazur L
Department of Animal Physiology, Jagiellonian University, Cracow, Poland.
Acta Physiol Hung. 1989;74(3-4):315-9.
Adult Swiss male mice were injected intraperitoneally with 2-aminoethylesothiouronium bromide hydrobromide (AET) or cysteamine hydrochloride (MEA) in a dose of 400 mg/kg body weight. In the thirtieth, sixtieth or ninetieth minute after the injection, the animals were killed and the deoxyribonuclein acid content in 100 mg of fresh tissue of testes, spleen and liver, was measured. DNA was extracted from the organs by means of Burton's method, which is based on the estimation of deoxiribose content in the colour reaction with diphenylamine. The injection of AET and MEA did not distinctly influence the DNA content in the organs of mice. Statistically significant differences among the groups of mice were not observed compared to the controls, in mice treated with the compound, a decreasing tendency in the quantity of the DNA in the organs was found only.
成年瑞士雄性小鼠腹腔注射氢溴酸2-氨基乙基异硫脲（AET）或盐酸半胱胺（MEA），剂量为400mg/kg体重。注射后第30、60或90分钟，处死动物，测定睾丸、脾脏和肝脏100mg新鲜组织中的脱氧核糖核酸含量。采用伯顿法从器官中提取DNA，该方法基于在与二苯胺的显色反应中对脱氧核糖含量的估算。注射AET和MEA对小鼠器官中的DNA含量没有明显影响。与对照组相比，未观察到各小鼠组之间有统计学显著差异，在用该化合物处理的小鼠中，仅发现器官中DNA数量有下降趋势。
