Mazur L, Manowska J
Department of Animal Physiology, Jagiellonian University, Cracow, Poland.
Strahlenther Onkol. 1990 Nov;166(11):764-7.
The adult male Swiss mice were either whole-body 60Co gamma-irradiated with a single dose of 10 Gy at 1900, or treated intraperitoneally with AET (2-aminoethylisothiouronium bromide hydrobromide) or MEA (cysteamine hydrochloride), in a dose of 400 mg/kg body weight, 15 minutes before irradiation. The measurements of the arylsulphatase (E.C. 3.1.6.1) activity in crude homogenate of liver were done every four hours during 24-hour period, starting at 2000. The enzyme activity towards the artificial substrate p-nitrocatechol sulphate was related to 1 mg of protein, 1 g of fresh tissue and to the whole organ weight. No fluctuations in the activity of arylsulphatase in the control mice were found. There were temporary changes in the enzyme activity expressed in nkat per 1 mg of protein and 1 g of fresh tissue in the irradiated only males, in that related to 1 g of fresh tissue and the whole organ weight in mice injected with AET before irradiation, as well as that calculated in nkat per 1 mg of protein in animals pre-treated with MEA to exposure of gamma rays. Between the particular groups of males, differences in the arylsulphatase activity related to 1 g of fresh tissue and the whole liver weight at 0400 were only observed. 60Co gamma-irradiation influenced the activity of arylsulphatase in liver. AET and MEA modified the effect of exposure of mice to gamma rays on the enzyme activity in a different way.
成年雄性瑞士小鼠，一组于19:00接受单次10 Gy的全身60Coγ射线照射，另一组在照射前15分钟腹腔注射400 mg/kg体重的AET（氢溴酸2-氨基乙基异硫脲）或MEA（盐酸半胱胺）。从20:00开始，在24小时内每4小时对肝脏粗匀浆中的芳基硫酸酯酶（E.C. 3.1.6.1）活性进行一次测定。以对人工底物硫酸对硝基邻苯二酚的酶活性与1 mg蛋白质、1 g新鲜组织及整个器官重量的关系来表示。对照组小鼠未发现芳基硫酸酯酶活性有波动。仅接受照射的雄性小鼠中，以每1 mg蛋白质和1 g新鲜组织中nkat表示的酶活性有暂时变化；照射前注射AET的小鼠中，以每1 g新鲜组织和整个器官重量表示的酶活性有暂时变化；预先用MEA处理后再接受γ射线照射的动物中，以每1 mg蛋白质中nkat计算的酶活性有暂时变化。仅在特定组别的雄性小鼠中，观察到在04:00时，与1 g新鲜组织及整个肝脏重量相关的芳基硫酸酯酶活性存在差异。60Coγ射线照射影响肝脏中芳基硫酸酯酶的活性。AET和MEA以不同方式改变了小鼠受γ射线照射对该酶活性的影响。
