Warner Judith E A, Larson Alexander J, Bhosale Prakash, Digre Kathleen B, Henley Courtney, Alder Stephen C, Katz Bradley J, Bernstein Paul S
Department of Ophthalmology and Visual Sciences, University of Utah Health Sciences Center, Salt Lake City, Utah 84132, USA.
J Neuroophthalmol. 2007 Dec;27(4):258-62. doi: 10.1097/WNO.0b013e31815b9af0.
Several studies have implicated vitamin A-related compounds in the pathogenesis of idiopathic intracranial hypertension (IIH). The goal of this study was to compare cerebrospinal fluid (CSF) and serum concentrations of retinol and retinol-binding protein (RBP) in subjects with and without IIH.
CSF and serum samples were collected from 87 subjects. The study population was composed of subjects with IIH (IIH group, n = 28), subjects with non-IIH neurologic conditions (neurology controls, n = 42), and subjects undergoing preoperative lumbar puncture but with no known neurologic conditions (anesthesia controls, n = 17). RBP levels (nM) were determined using radial immunodiffusion, and retinol levels (nM) were determined using high-performance liquid chromatography.
The retinol/RBP ratio was greater in CSF than in serum, especially in subjects with IIH.
The finding of increased levels of unbound retinol in the CSF of subjects with IIH provides further evidence that vitamin A may be involved in the pathogenesis of IIH. Comparative statistical analyses revealed multivariate relationships that demonstrate the need to further investigate correlations between vitamin A and RBP levels in CSF and serum.
多项研究表明维生素A相关化合物与特发性颅内高压（IIH）的发病机制有关。本研究的目的是比较患有和未患有IIH的受试者脑脊液（CSF）和血清中视黄醇及视黄醇结合蛋白（RBP）的浓度。
从87名受试者中采集脑脊液和血清样本。研究人群包括患有IIH的受试者（IIH组，n = 28）、患有非IIH神经系统疾病的受试者（神经科对照组，n = 42）以及接受术前腰椎穿刺但无已知神经系统疾病的受试者（麻醉对照组，n = 17）。使用放射免疫扩散法测定RBP水平（nM），使用高效液相色谱法测定视黄醇水平（nM）。
脑脊液中的视黄醇/RBP比值高于血清，尤其是在患有IIH的受试者中。
IIH受试者脑脊液中游离视黄醇水平升高的发现进一步证明维生素A可能参与IIH的发病机制。比较统计分析揭示了多变量关系，表明需要进一步研究脑脊液和血清中维生素A与RBP水平之间的相关性。
