Xing Liyu, Franz Michael G, Marcelo Cynthia L, Smith Charlotte A, Marshall Vivienne S, Robson Martin C
Department of Surgery, University of Michigan, Ann Arbor, MI, USA.
J Burns Wounds. 2007 Oct 5;7:e5.
Acute wound failure is a common complication following surgical procedures and trauma. Laparotomy wound failure leads to abdominal dehiscence and incisional hernia formation. Delayed recovery of wound-breaking strength is one mechanism for laparotomy wound failure. Early fascial wounds are relatively acellular, and there is a delay in the appearance of acute wound growth factors and cytokines. The objective of this study was to accelerate and improve laparotomy wound healing using amnion-derived multipotent cells (AMPs). AMPs' nonimmunogenic phenotype and relative abundance support its role as a cell therapy.
AMPs were injected into the load-bearing layer of rat abdominal walls prior to laparotomy, and cell viability was confirmed. Wound mechanical properties were measured over 28 days. The incidence and severity of laparotomy wound failure was measured in an incisional hernia model.
AMP cells were viable in laparotomy wounds for at least 28 days and did not migrate to other tissues. Laparotomy wound-breaking strength was increased by postoperative day 7 following AMP therapy. AMP therapy reduced the incidence of hernia formation and the size of hernia defects. Histology suggested stimulated wound fibroplasia and angiogenesis.
AMP cell therapy reduces the incidence of laparotomy wound failure by accelerating the recovery of wound-breaking strength. This results in fewer incisional hernias and smaller hernia defects.
急性伤口愈合不良是外科手术和创伤后常见的并发症。剖腹手术伤口愈合不良会导致腹部切口裂开和切口疝形成。伤口抗张强度恢复延迟是剖腹手术伤口愈合不良的一种机制。早期筋膜伤口相对无细胞，急性伤口生长因子和细胞因子的出现也会延迟。本研究的目的是使用羊膜来源的多能细胞（AMPs）加速并改善剖腹手术伤口的愈合。AMPs的非免疫原性表型和相对丰富性支持其作为一种细胞疗法的作用。
在剖腹手术前将AMPs注入大鼠腹壁的承重层，并确认细胞活力。在28天内测量伤口的力学性能。在切口疝模型中测量剖腹手术伤口愈合不良的发生率和严重程度。
AMPs细胞在剖腹手术伤口中存活至少28天，且未迁移至其他组织。AMPs治疗后，术后第7天剖腹手术伤口的抗张强度增加。AMPs治疗降低了疝形成的发生率和疝缺损的大小。组织学检查提示伤口成纤维细胞增生和血管生成受到刺激。
AMPs细胞疗法通过加速伤口抗张强度的恢复降低了剖腹手术伤口愈合不良的发生率。这导致切口疝减少，疝缺损变小。
