Handa Nobuhiro, Magata Yasuhiro, Mukai Takahiro, Nishina Takeshi, Konishi Junji, Komeda Masashi
Department of Cardiovascular Surgery, Kyoto University Graduate School of Medicine, 54 Kawaharacho, Shogoin, Sakyo-ku, Kyoto, 606-8507, Japan.
Ann Nucl Med. 2007 Dec;21(10):569-76. doi: 10.1007/s12149-007-0067-2. Epub 2007 Dec 25.
Quantitative myocardial fluorodeoxyglucose positron emission tomography (FDG-PET) for assessing glucose uptake in vivo is reliable in normal rat heart.
To assess the applicability of myocardial FDG-PET on multiple occasions in the longitudinal disease process of progressive hypertrophy of rat heart.
Six salt-sensitive Dahl rats (Dahl-S) developing progressive hypertrophy with subsequent dilated cardiomyopathy were compared with salt-resistant Dahl rats (controls). FDG-PET was applied twice at early stage (ES: 14-18 weeks) and at late stage (LS: 22-26 weeks) of hypertrophy. Standardized uptake value (SUV) was calculated for comparing between different animal weights and different injection dosages of FDG. For validating the quantitative study, radioactivity of a total of 36 tissue samples was compared with the corresponding PET values.
The left ventricular mass in Dahl-S increased by 17% at ES and by 25% at LS. The SUV in Dahl-S was 95% of controls at ES and reduced to 62% at LS (P=0.023). The heart function started to deteriorate after LS. Linear regression analysis showed a good correlation between the radioactivity of tissue samples and PET values (Y=1.20X, P<0.0001, R2=0.979).
Small animal PET studies on longitudinal multiple occasions in vivo were feasible and useful for the repeating assessment of glucose uptake. The reduction of glucose uptake in progressive hypertrophy of heart over time may precede its progression to heart failure.
定量心肌氟脱氧葡萄糖正电子发射断层扫描（FDG-PET）用于评估体内葡萄糖摄取在正常大鼠心脏中是可靠的。
评估心肌FDG-PET在大鼠心脏进行性肥大的纵向疾病过程中多次应用的适用性。
将6只发生进行性肥大并随后发展为扩张型心肌病的盐敏感Dahl大鼠（Dahl-S）与盐抵抗Dahl大鼠（对照组）进行比较。在肥大的早期（ES：14 - 18周）和晚期（LS：22 - 26周）两次应用FDG-PET。计算标准化摄取值（SUV）以比较不同动物体重和不同FDG注射剂量之间的差异。为验证定量研究，将总共36个组织样本的放射性与相应的PET值进行比较。
Dahl-S大鼠的左心室质量在ES时增加了17%，在LS时增加了25%。Dahl-S大鼠在ES时的SUV是对照组的95%，在LS时降至62%（P = 0.023）。心脏功能在LS后开始恶化。线性回归分析显示组织样本的放射性与PET值之间具有良好的相关性（Y = 1.20X，P < 0.0001，R2 = 0.979）。
小动物体内纵向多次PET研究对于重复评估葡萄糖摄取是可行且有用的。心脏进行性肥大过程中葡萄糖摄取随时间的减少可能先于其发展为心力衰竭。
