Prado Cibele M, Fine Eugene J, Koba Wade, Zhao Dazhi, Rossi Marcos A, Tanowitz Herbert B, Jelicks Linda A
Department of Pathology, University of São Paulo, Ribeirão Preto, Brazil.
Am J Trop Med Hyg. 2009 Nov;81(5):900-5. doi: 10.4269/ajtmh.2009.09-0338.
Noninvasive assessment of cardiac structure and function is essential to understand the natural course of murine infection with Trypanosoma cruzi. Magnetic resonance imaging (MRI) and echocardiography have been used to monitor anatomy and function; positron emission tomography (PET) is ideal for monitoring metabolic events in the myocardium. Mice infected with T. cruzi (Brazil strain) were imaged 15-100 days post infection (dpi). Quantitative (18)F-FDG microPET imaging, MRI and echocardiography were performed and compared. Tracer ((18)F-FDG) uptake was significantly higher in infected mice at all days of infection, from 15 to 100 dpi. Dilatation of the right ventricular chamber was observed by MRI from 30 to 100 dpi in infected mice. Echocardiography revealed significantly reduced ejection fraction by 60 dpi. Combination of these three complementary imaging modalities makes it possible to noninvasively quantify cardiovascular function, morphology, and metabolism from the earliest days of infection through the chronic phase.
对心脏结构和功能进行无创评估对于了解克氏锥虫感染小鼠的自然病程至关重要。磁共振成像（MRI）和超声心动图已用于监测心脏解剖结构和功能；正电子发射断层扫描（PET）是监测心肌代谢事件的理想方法。对感染克氏锥虫（巴西株）的小鼠在感染后15 - 100天进行成像。进行了定量（18）F - FDG微PET成像、MRI和超声心动图检查并进行比较。在感染后的所有天数，即从感染后15天到100天，感染小鼠体内示踪剂（（18）F - FDG）摄取均显著更高。通过MRI观察到感染小鼠在感染后30天至100天右心室腔扩张。超声心动图显示在感染后60天射血分数显著降低。这三种互补成像方式的结合使得从感染最早阶段到慢性期对心血管功能、形态和代谢进行无创定量成为可能。
