Noguerol-Cal R, López-Vilariño J M, Fernández-Martínez G, Barral-Losada L, González-Rodríguez M V
Laboratorio de Química, Centro de Investigacións Tecnolóxicas, UDC-Campus de Esteiro s/n, 15403 Ferrol, Spain.
J Chromatogr A. 2008 Feb 1;1179(2):152-60. doi: 10.1016/j.chroma.2007.11.099. Epub 2007 Dec 3.
Two high-performance liquid chromatographic methods, with ultraviolet-visible spectrophotometry detection (HPLC-UV/Vis) and with tandem mass spectrometry triple quadrupole interfaced with positive ion mode electrospray ionization detection (HPLC-ESI+-QqQ-MS/MS), for determination and quantification of ten commercial dyes are proposed for control in commercial products. Multiple peaks observed for some of the studied dyes in HPLC-UV/Vis chromatograms forced to obtain structural information by HPLC-ESI-MS/MS method with scan mode. The quality parameters of the two proposed chromatographic methods were evaluated for different requirements of normative, showing detection (LODs) and quantification (LOQs) limits around 60-890 and 200-2990 microg L(-1) for HPLC-UV/Vis, and 4.54-14.3 and 15.0-47.6 microg L(-1) for HPLC-ESI+-QqQ-MS/MS.
本文提出了两种高效液相色谱法，分别采用紫外-可见分光光度检测法（HPLC-UV/Vis）和串联质谱三重四极杆正离子模式电喷雾电离检测法（HPLC-ESI+-QqQ-MS/MS），用于测定和定量十种商用染料，以控制商业产品质量。在HPLC-UV/Vis色谱图中，部分被测染料出现多个峰，因此需采用扫描模式的HPLC-ESI-MS/MS方法获取结构信息。针对不同的规范要求，评估了这两种色谱方法的质量参数，结果表明，HPLC-UV/Vis的检测限（LOD）和定量限（LOQ）分别在60-890和200-2990 μg L(-1)左右，而HPLC-ESI+-QqQ-MS/MS的检测限和定量限分别为4.54-14.3和15.0-47.6 μg L(-1)。
