Kaou Ali Mohamed, Mahiou-Leddet Valérie, Hutter Sébastien, Aïnouddine Sidi, Hassani Said, Yahaya Ibrahim, Azas Nadine, Ollivier Evelyne
Laboratoire de Pharmacognosie, EA864, Faculté de Pharmacie, Université de la Méditerranée (Aix-Marseille II), 13385 Marseille, Cedex 5, France.
J Ethnopharmacol. 2008 Feb 28;116(1):74-83. doi: 10.1016/j.jep.2007.11.001. Epub 2007 Nov 9.
An ethnobotanical study was conducted in Comores (Ngazidja) about plant species used traditionally for the treatment of various diseases, including malaria. Antimalarial activity of 76 vegetal extracts obtained from 17 species traditionally used to treat malaria symptoms, was evaluated in vitro using Plasmodium falciparum chloroquine-resistant strain (W2). Antiproliferative activity was evaluated on human monocytic THP1 cells and the selectivity index of the plant extracts was calculated. The results showed that 10 plant extracts had a moderate activity (5<IC(50)< or =10 microg/ml), and 6 a good in vitro activity with IC(50) value < or =5 microg/ml. The highest antiplasmodial activity was found for the MeOH/H(2)O leaves extract of Flueggea virosa (Roxb. Ex Willd.) Voigt subsp. virosa (Euphorbiaceae) (IC(50)=2 microg/ml), for roots decoction of Flueggea virosa (IC(50)=3 microg/ml) and for chloromethylenic roots extract of Vernonia colorata (Willd.) Drake subsp. grandis (DC.) C. Jeffrey (Asteraceae) (IC(50)=3 microg/ml). Three other extracts showed moderate antiplasmodial activity (IC(50)<5 microg/ml): Vernonia colorata (aerial part), Piper capense L.f. (Piperaceae), and Leptadenia madagascariensis Decne (Asclepiadaceae) chloromethylenic extracts (IC(50)=6 microg/ml; 7 microg/ml and 9 microg/ml, respectively). All the plants tested displayed a low cytotoxicity on THP1 cells.
在科摩罗（恩加齐贾岛）开展了一项民族植物学研究，以探究传统上用于治疗包括疟疾在内的各种疾病的植物种类。从17种传统上用于治疗疟疾症状的植物中提取了76种植物提取物，利用恶性疟原虫氯喹抗性株（W2）在体外评估其抗疟活性。在人单核细胞THP1细胞上评估其抗增殖活性，并计算植物提取物的选择性指数。结果表明，10种植物提取物具有中等活性（5＜半数抑制浓度（IC50）≤10微克/毫升），6种具有良好的体外活性，IC50值≤5微克/毫升。发现白饭树（大戟科）的甲醇/水叶提取物（IC50 = 2微克/毫升）、白饭树根煎剂（IC50 = 3微克/毫升）以及紫斑鸠菊（菊科）氯甲基化根提取物（IC50 = 3微克/毫升）的抗疟活性最高。其他三种提取物显示出中等抗疟活性（IC50＜5微克/毫升）：紫斑鸠菊（地上部分）、卡彭氏胡椒（胡椒科）以及马达加斯加细叶腺果藤（萝藦科）氯甲基化提取物（IC50分别为6微克/毫升、7微克/毫升和9微克/毫升）。所有测试植物对THP1细胞均显示出低细胞毒性。
