Pan Suhua, Yu Qian, Bie Mingjiang, Liu Xiang
Panyu Entry-Exit Inspection And Quarantine Bureau, Guangzhou 511400, China.
Wei Sheng Yan Jiu. 2007 Sep;36(5):618-21.
To investigate the adherence ability of four Lactobacilli to gastric epithelial cell SGC-7901 and the ability of inhibiting the adherence of Helicobacter pylori to the cells.
The light microscope and scanning electron microscope were used to detect Lactobacilli that adhering to SGC-7901 cells after co-culturing for 2 hours. Using FITC-conjuncted antibody, the adhered Helicobacter pylori density was evaluated under fluorescence microscope after pretreatment with live or nonviable Lactobacilli.
Four Lactobacilli's live and nonviable stains could adhere to SGC-7901 tightly and obviously inhibit the adherence of Helicobacter pylori, but their adherence ability was not different, excluding their live stains adherence ability. And their inhibiting ability were different, of which the best was Lac15, the worst was Lac12.
Four Lactobacilli can inhibit the adherence of Helicobacter pylori to gastric epithelial cell SGC-7901 in vitro, and they should be further evaluated in vivo.
研究4株乳酸杆菌对胃上皮细胞SGC-7901的黏附能力以及抑制幽门螺杆菌对该细胞黏附的能力。
采用光学显微镜和扫描电子显微镜检测共培养2小时后黏附于SGC-7901细胞的乳酸杆菌。在用活的或灭活的乳酸杆菌预处理后，使用异硫氰酸荧光素标记的抗体，在荧光显微镜下评估黏附的幽门螺杆菌密度。
4株乳酸杆菌的活菌和死菌均可紧密黏附于SGC-7901，并明显抑制幽门螺杆菌的黏附，但它们的黏附能力无差异（不包括活菌的黏附能力）。它们的抑制能力不同，其中Lac15最佳，Lac12最差。
4株乳酸杆菌在体外可抑制幽门螺杆菌对胃上皮细胞SGC-7901的黏附，应进一步进行体内评估。
