山梨醇操纵子调节因子SorC四聚体形式的过表达、纯化及结晶
Overexpression, purification and crystallization of the tetrameric form of SorC sorbitol operon regulator.
作者信息
de Sanctis Daniele, Rêgo Ana T, Marçal David, McVey Colin E, Carrondo Maria A, Enguita Francisco J
机构信息
Instituto de Tecnologia Química e Biológica, Universidade Nova de Lisboa, Avenida da República, Apartado 127, 2781-901 Oeiras, Portugal.
出版信息
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2008 Jan 1;64(Pt 1):22-4. doi: 10.1107/S1744309107060897. Epub 2007 Dec 20.
Abstract
The sorbitol operon regulator (SorC) regulates the metabolism of L-sorbose in Klebsiella pneumonia. SorC was overexpressed in Escherichia coli and purified, and crystals were obtained of a tetrameric form. A single crystal showed X-ray diffraction to 3.20 A. The crystal belongs to space group P2(1)2(1)2(1), with unit-cell parameters a = 91.6, b = 113.3, c = 184.1 A. Analysis of the molecular-replacement solution indicates the presence of four SorC molecules in the asymmetric unit.
摘要
山梨醇操纵子调节因子（SorC）调节肺炎克雷伯菌中L-山梨糖的代谢。SorC在大肠杆菌中过表达并纯化，获得了四聚体形式的晶体。一颗单晶的X射线衍射分辨率达到3.20 Å。该晶体属于空间群P2(1)2(1)2(1)，晶胞参数为a = 91.6、b = 113.3、c = 184.1 Å。分子置换解的分析表明不对称单元中存在四个SorC分子。
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