Walker Moira S, Hughes Thomas A
YCR and Liz Dawn Pathology and Translational Sciences Centre, Leeds Institute of Molecular Medicine, St James's University Hospital, University of Leeds, Leeds LS9 7TF, UK.
Int J Mol Med. 2008 Jan;21(1):13-7.
Analysis of the transcriptome using DNA microarrays has become a standard approach for investigation of the molecular basis of human disease in both clinical and experimental settings. However, drawing conclusions from the wealth of data obtained has remained problematic. There have been difficulties with accurate reporting of results, with experimental reproducibility and with identifying and interpreting the biologically relevant information. In this review we discuss the successful use of DNA microarray technology in molecular medical research, and we highlight methods of addressing the issues of both reproducibility and biological interpretation.
利用DNA微阵列分析转录组已成为在临床和实验环境中研究人类疾病分子基础的标准方法。然而，从所获得的大量数据中得出结论仍然存在问题。在结果的准确报告、实验可重复性以及识别和解释生物学相关信息方面都存在困难。在这篇综述中，我们讨论了DNA微阵列技术在分子医学研究中的成功应用，并强调了解决可重复性和生物学解释问题的方法。
