Gaudin R, Marsan P, Robert A, Ducos P, Pruvost A, Lévi M, Bouscaillou P
Département Polluants et Santé, Institut National de Recherche et de Sécurité, Vandoeuvre Cedex, France.
Int Arch Occup Environ Health. 2008 Aug;81(8):959-66. doi: 10.1007/s00420-007-0289-6. Epub 2007 Dec 21.
The aim of this study was to perform a biological monitoring survey of workers exposed to di(2-ethylhexyl)phthalate (DEHP) in a factory using polyvinyl chloride plastisols and to contribute additional occupational data of exposure particularly sparse in the industrial sectors where this plasticizer is used.
Three urinary metabolites of DEHP, mono(2-ethylhexyl)phthalate (MEHP), mono(5-carboxy-2-ethylpentyl)phthalate (MCEPP) and 2-ethylhexanoic acid (2-EHA) were quantified in five workers using a plastisol (containing 33% of DEHP) and in five unexposed workers (controls), during 5 days with pre- and post-shift sampling. Analyses were performed by high-performance liquid chromatography with tandem mass spectrometry (HPLC-MS/MS) with on-line extraction.
Median concentrations of pre- and post-shift urinary samples in the exposed workers (n = 25) were 16.1 and 55.9 microg/l for MEHP, 37.6 and 103.7 microg/l for MCEPP and 46.3 and 72.1 microg/l for 2-EHA, respectively. In the controls (n = 19), the corresponding values were 12.0 and 10.4 microg/l for MEHP, 38.1 and 11.4 microg/l for MCEPP and 31.9 and 46.0 microg/l for 2-EHA, respectively. There is a significant increase (Mann-Whitney U-test, P < 0.05) of post-shift excretion in the exposed workers versus unexposed controls and in post-shift versus pre-shift concentrations only in the exposed workers.
MEHP and MCEPP are shown to be suitable biomarkers to assess DEHP exposure while 2-EHA, less specific but classified in the category 3 of the European Union (EU) reproductive toxicants, is also an interesting biomarker. There is clear evidence of occupational exposure of workers in this factory.
本研究旨在对一家使用聚氯乙烯增塑溶胶的工厂中接触邻苯二甲酸二（2-乙基己基）酯（DEHP）的工人进行生物监测调查，并提供有关这种增塑剂使用行业中特别稀少的额外职业暴露数据。
在5天的时间里，对5名使用增塑溶胶（含33%DEHP）的工人和5名未接触者（对照组）进行班前和班后采样，使用高效液相色谱-串联质谱法（HPLC-MS/MS）在线萃取对DEHP的三种尿代谢物邻苯二甲酸单（2-乙基己基）酯（MEHP）、邻苯二甲酸单（5-羧基-2-乙基戊基）酯（MCEPP）和2-乙基己酸（2-EHA）进行定量分析。
接触组（n = 25）班前和班后尿样中MEHP的中位数浓度分别为16.1和55.9微克/升，MCEPP分别为37.6和103.7微克/升，2-EHA分别为46.3和72.1微克/升。对照组（n = 19）中，MEHP的相应值分别为12.0和10.4微克/升，MCEPP分别为38.1和11.4微克/升，2-EHA分别为31.9和46.0微克/升。与未接触对照组相比，接触组工人班后排泄量显著增加（曼-惠特尼U检验，P < 0.05），且仅在接触组中，班后浓度相对于班前浓度有显著增加。
MEHP和MCEPP被证明是评估DEHP暴露的合适生物标志物，而2-EHA虽然特异性较低，但属于欧盟生殖毒物3类，也是一个有趣的生物标志物。有明确证据表明该工厂工人存在职业暴露。
