Netesova N A, Muravlev A I, Chikaev N A
Mol Gen Mikrobiol Virusol. 1989 Nov(11):21-3.
The left HindIII-A-Sal fragment of the vaccinia virus DNA has been analyzed by the technique of mRNA hybridizational selection with the subsequent translation in cell-free protein-synthesizing system from the rabbit reticulocytes. The viral mRNA hybridizable with the fragment was shown to direct the synthesis of 12, 17, 27, 42, 70 kD polypeptides in the cell-free protein-synthesizing system. Each of 12 and 42 kD polypeptides was demonstrated to react specifically with antisera to structural p12 and p42 coat proteins. The structural coat proteins p12, p20, p42 of the vaccinia virus are concluded to be the products of the same viral gene.
通过mRNA杂交选择技术，并随后在兔网织红细胞的无细胞蛋白质合成系统中进行翻译，对痘苗病毒DNA的左HindIII-A-Sal片段进行了分析。结果表明，可与该片段杂交的病毒mRNA在无细胞蛋白质合成系统中指导合成12、17、27、42、70 kD的多肽。已证明12 kD和42 kD的每种多肽都能与针对结构p12和p42衣壳蛋白的抗血清发生特异性反应。得出结论，痘苗病毒的结构衣壳蛋白p12、p20、p42是同一病毒基因的产物。
