Skreb N, Bulić F
Dev Biol. 1987 Apr;120(2):584-6. doi: 10.1016/0012-1606(87)90261-2.
Modified organ cultures of rat egg cylinders were grown for 2 weeks in Eagle's MEM without serum or with serum added at different times. Explant survival was decreased only in cultures grown for the entire 2 weeks in serum-free medium, whereas the explant growth was impeded in all but the cultures grown for 2 weeks in 50% MEM plus 50% serum. Differentiation of epidermis and cartilage in the cultures deprived of serum for the entire 2-week period was comparable to that in fully serum-supplemented medium, whereas other differentiated tissues were rare or absent. In explants cultivated without serum for only the first week, neuroblasts were scarce.
对大鼠卵圆柱进行改良器官培养，在不含血清的伊格尔氏基本培养基（MEM）中培养2周，或在不同时间添加血清进行培养。仅在无血清培养基中培养2周的培养物中，外植体存活率降低，而除了在50% MEM加50%血清中培养2周的培养物外，所有培养物中的外植体生长均受到阻碍。在整个2周期间无血清培养的培养物中，表皮和软骨的分化与完全补充血清的培养基中的分化相当，而其他分化组织很少或不存在。在仅第一周无血清培养的外植体中，神经母细胞很少。
