Venkatesan Jayachandran, Manivasagan Panchanathan, Kim Se-Kwon, Kirthi Arivarasan Vishnu, Marimuthu Sampath, Rahuman Abdul Abdul
Marine Bioprocess Research Center, Pukyong National University, 599-1 Daeyeon 3-dong, Nam-gu, Pusan, 608-737, Republic of Korea.
Bioprocess Biosyst Eng. 2014 Aug;37(8):1591-7. doi: 10.1007/s00449-014-1131-7. Epub 2014 Feb 14.
In the present study, we report rapid biological synthesis of gold nanoparticles (Au NPs) using a novel marine brown alga Ecklonia cava (Family: Lessoniaceae) by the reduction of chloroauric acid. The formation of Au NPs reaction was complete within 1 min at 80 °C and physiochemically characterized with different analytical techniques. FTIR spectroscopy revealed that Au NPs were functionalized with biomolecules that have primary amine group, hydroxyl group and other stabilizing functional groups. X-ray diffraction pattern showed high purity and face-centered cubic structure of Au NPs. Microscopy results showed that these Au NPs are formed with shapes like spherical and triangular with an average size of 30 ± 0.25 nm. Synthesized Au NPs showed good antimicrobial and biocompatibility with human keratinocyte cell line. Thus, physiochemical characteristic results suggest that Au NPs will have promising biomedical applications in different area such as drug delivery, tissue engineering, biosensor, etc.
在本研究中，我们报告了使用一种新型海洋褐藻——凹顶藻（海带科）通过氯金酸还原法快速生物合成金纳米颗粒（Au NPs）。Au NPs的形成反应在80℃下1分钟内完成，并使用不同的分析技术对其进行了物理化学表征。傅里叶变换红外光谱显示，Au NPs被具有伯胺基、羟基和其他稳定官能团的生物分子功能化。X射线衍射图谱显示Au NPs具有高纯度和面心立方结构。显微镜结果表明，这些Au NPs的形状为球形和三角形，平均尺寸为30±0.25nm。合成的Au NPs对人角质形成细胞系显示出良好的抗菌性和生物相容性。因此，物理化学特性结果表明，Au NPs在药物递送、组织工程、生物传感器等不同领域将具有广阔的生物医学应用前景。
