Mendez Susana, Hatem Christine L, Kesavan Anup K, Lopez-Molina Javier, Pitt M Louise M, Dannenberg Arthur M, Manabe Yukari C
JA Baker Institute for Animal Health, Cornell Veterinary College, Ithaca, NY 14853, USA.
Vet Immunol Immunopathol. 2008 Mar 15;122(1-2):167-74. doi: 10.1016/j.vetimm.2007.11.006. Epub 2007 Nov 17.
We sought to characterize the lung cellular immune responses to inhaled Mycobacterium tuberculosis (Mtb) of the susceptible inbred Thorbecke rabbit (the genomically sequenced strain, now unavailable) and compare it to outbred, Mtb-resistant, New Zealand White rabbits. Using Mtb CDC1551, we confirmed that the inbred rabbits allowed establishment of infection with this low virulence strain, compared to poor establishment in outbred rabbits. With a more virulent strain, Mtb Erdman, that establishes infection well in both rabbit strains, we analyzed granulomas from rabbit lungs 5 weeks after aerosol infection. The lung granulomas of inbred rabbits had significantly higher frequencies of cells expressing MHC Class II and CD11b, and lower frequencies of CD8+ T cells than the outbred controls. Macrophage-sized cells expressing MHC Class II in inbred rabbit granulomas showed significantly decreased intensity of expression, suggesting impaired maturation. Although the inbred dermal tuberculin reactions were decreased, the in vitro IFN-gamma mRNA responses of hilar node lymphocytes to tuberculin were higher than those of outbred rabbits. Further delineation of the outbred rabbit's resistant immune response to Mtb infection is warranted.
我们试图描述易感性近交系索尔贝克兔（基因组已测序的品系，现已无法获得）对吸入结核分枝杆菌（Mtb）的肺部细胞免疫反应，并将其与远交系、抗Mtb的新西兰白兔进行比较。使用Mtb CDC1551，我们证实近交系兔子能够感染这种低毒力菌株，而远交系兔子则难以建立感染。对于在两种兔品系中都能很好地建立感染的更强毒力菌株Mtb Erdman，我们分析了气溶胶感染5周后兔肺中的肉芽肿。与远交系对照相比，近交系兔子的肺肉芽肿中表达MHC II类分子和CD11b的细胞频率显著更高，而CD8+ T细胞频率更低。近交系兔肉芽肿中表达MHC II类分子的巨噬细胞大小的细胞显示出显著降低的表达强度，表明其成熟受损。尽管近交系的皮肤结核菌素反应降低，但肺门淋巴结淋巴细胞对结核菌素的体外IFN-γ mRNA反应高于远交系兔子。有必要进一步阐明远交系兔子对Mtb感染的抗性免疫反应。
