Caffesse R G, Nasjleti C E
J Periodontol. 1976 Jul;47(7):391-7. doi: 10.1902/jop.1976.47.7.391.
To explore possible enzymatic penetration through intact sulcular epithelium tritium labeled hyaluronidase and collagenase were applied to the gingival sulcus of five white lip marmosets. One quadrant per monkey remained untreated. Each remaining quadrant was randomly assigned to one of the following modalities of application: (a) tritiated hyaluronidase, (b) tritiated collagenase, (c) unlabeled hyaluronidase followed by tritiated collagenase, (d) inactivated tritiated hyaluronidase, or (e) normal saline as experimental controls. The enzymes were applied by means of a Pasteur disposable pipette. Eight drops were administered over a 4-minute period, one every 30 seconds. After a 5-minute waiting period a second series of eight applications was given over a 4-minute period. Radioautographic and standard histologic materials were obtained. Results suggest that: 1. Hyaluronidase has the ability to penetrate through intact nonkeratinized sulcular epithelium, widening the intercellular epithelial spaces and disorganizing the connective tissue ground substance. 2. Collagenase per se does not have the ability to penetrate through the intact sulcular epithelium. Its effect remains confined only to the superficial layers of the epithelium. 3. However, when collagenase application is preceded by hyaluronidase, collagenase spreads easily through the epithelium and deeply into the connective tissue. Hyaluronidase acts unquestionably as a spreading factors.
为了探究酶是否有可能穿透完整的龈沟上皮，将氚标记的透明质酸酶和胶原酶应用于五只白唇狨猴的龈沟。每只猴子有一个象限未接受处理。其余每个象限被随机分配到以下应用方式之一：(a) 氚标记的透明质酸酶，(b) 氚标记的胶原酶，(c) 未标记的透明质酸酶后接氚标记的胶原酶，(d) 灭活的氚标记透明质酸酶，或 (e) 生理盐水作为实验对照。通过巴斯德一次性移液管应用这些酶。在4分钟内滴入8滴，每30秒滴入一滴。经过5分钟的等待期后，在4分钟内进行第二轮8次滴注。获取了放射自显影和标准组织学材料。结果表明：1. 透明质酸酶有能力穿透完整的非角化龈沟上皮，扩大细胞间上皮间隙并破坏结缔组织基质。2. 胶原酶本身没有能力穿透完整的龈沟上皮。其作用仅局限于上皮的表层。3. 然而，当在应用胶原酶之前先应用透明质酸酶时，胶原酶很容易穿过上皮并深入结缔组织。透明质酸酶无疑起到了扩散因子的作用。
