Gaffar A, Coleman E J, Marcussen H W
J Periodontol. 1981 Apr;52(4):197-205. doi: 10.1902/jop.1981.52.4.197.
EIGHTY-FIVE Sprague-Dawley rats were used in two experiments to determine the conditions necessary to permit transepithelial penetration by deleterious macromolecules in murine oral mucosa. In experiment one, Group I was a water and diet control; Group II mucosa was treated with hyaluronidase; Group III with streptococcal polysaccharide; and Group IV with hyaluronidase, followed by treatment with the polysaccharide. In the second experiment, the histological effects of the streptococcal polysaccharide were quantified by administering a series of concentrations, from 10 mg/ml to 100 microgram/ml. The results suggest that tissue-damaging plaque components, such as hyaluronidase and polysaccharide, act in combination to pass through the epithelial structures into the subjacent connective tissues to cause destructive changes in rat gingiva. Such changes may possibly be related to those seen in the periodontium when it is adjacent to dental plaque.
在两项实验中使用了85只斯普拉格-道利大鼠，以确定有害大分子在鼠口腔黏膜中实现跨上皮渗透所需的条件。在实验一中，第一组为水和饮食对照组；第二组黏膜用透明质酸酶处理；第三组用链球菌多糖处理；第四组先用透明质酸酶处理，然后用多糖处理。在第二项实验中，通过给予一系列浓度（从10毫克/毫升到100微克/毫升）的链球菌多糖来量化其组织学效应。结果表明，诸如透明质酸酶和多糖等破坏组织的菌斑成分共同作用，穿过上皮结构进入下方的结缔组织，从而导致大鼠牙龈出现破坏性变化。这种变化可能与牙周组织靠近牙菌斑时所观察到的变化有关。
